Abstract
The immobilization of a whole microbial cell is an important process used in nanotechnology of biosensors and other related fields, especially the development of bio-hybrid materials based on live organisms and inorganic compounds. Here, we described an essay to develop a bio-hybrid material based on Salmonella Typhimurium cells and layered double hydroxides (LDH). The synthetic clays have a good capacity to be a host matrix for immobilization of live entity like bacteria. The incorporation of LDH in the nutritive broth shows the capacity of bacteria to grow under the inorganic conditions. The immobilization of bacteria onto the LDH Layer deposited on gold wafers was successfully done and the verification of the final material consistence was given by Fourier transform infrared spectroscopy (FTIR) analysis that shows the possibility of various covalent links that can be established between the polar functional group of the cell and the interlayer level in the LDH. The roughness of the surface was given by scanning electron microscope (SEM) imaging and shows the homogeneity of cell distribution on the LDH layer. Key words: Layer double hydroxide, Salmonella Typhimurium, Fourier transform infrared spectroscopy-attenuated total reflectance (FTIR-ATR), X-ray diffraction (XRD), energy dispersive X-ray (EDX); scanning electron microscope (SEM)
Highlights
The immobilization of enzymes for catalytic reactions coupled with redox reactions has gained an immense importance during the last two decades in the world of biosensors
The immobilization of bacteria onto the layered double hydroxides (LDH) Layer deposited on gold wafers was successfully done and the verification of the final material consistence was given by Fourier transform infrared spectroscopy (FTIR) analysis that shows the possibility of various covalent links that can be established between the polar functional group of the cell and the interlayer level in the LDH
To better explain the difference between the two LDH, elementary analysis have been done and the result are given in Table 2 which summarizes the crystalline parameter and confirms that the preparation of the LDH with co-precipitation synthesis method was successfully done according the study given by Hidouri et al (2011), Abdelkader et al (2011), Hidouri et al (2011) and Abdelkader et al (2011)
Summary
The immobilization of enzymes for catalytic reactions coupled with redox reactions has gained an immense importance during the last two decades in the world of biosensors. The coupling of bacteria with device transducer can convert a cellular response into detectable signals and make it possible to exploit the sensitivity of the cell to toxins and pollutants (Hua et al, 2015). The cell can retain most of its functionality and a wide range of enzymes will be protected in the cell, which can be exploited in several applications like detection or use for the synthesis of some substances for other applications, which can react with substrates outside the cell wall. Present as free forms, biomolecules such as enzymes, antibodies and receptors, presented in microorganisms as well as animal and plant cells or tissues have been used as biological sensing elements
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