Abstract
In this study, the transgenic sweet potato Xu55-2 modified with an engineered tandem repeat of a family 20 starch binding domain (SBD2) was analyzed by Western dot blot to investigate whether SBD2 proteins are capable of granule-targeting during starch biosynthesis. Furthermore, the impact of SBD2 accumulation in granules on the physicochemical properties of the transgenic starches was also investigated. Our results demonstrate that the high levels of SBD2 protein could be accumulated in granules. The SBD2 expression affect granule morphology without altering the primary structure of the constituent starch molecules, suggesting that SBD2 could be used as an anchor for effector proteins to sweet potato starch granules during biosynthesis. Key words: Sweet potato (Ipomoea batatas), tandem starch-binding domain, transgenic starch, granule morphology.
Highlights
Sweet potato (Ipomoea batatas) is one of the important starchy resources in Asia
The Tandem starch-binding domain (SBD2) accumulating clones were divided into four classes based on the amount of SBD2 protein associated with the starch granules, in which 0+, 1+, 2+ and 3+ represent no, low, intermediate and high accumulation levels, respectively (Figure 1A)
The results clearly show that the highest level of SBD2 accumulation in sweet potato starch granules was the 3+ class
Summary
Sweet potato (Ipomoea batatas) is one of the important starchy resources in Asia. It accounts for over 80% of world sweet potato starch production, most of which are in China. Over 10% of the production of 100 million tons of sweet potato in China is processed into starch. The use of sweet potato starch is primarily determined by its physicchemical properties. There are almost no natural starches with essential properties for a particular application. Different modifications must be made before applying natural starch
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