Abstract
To investigate the role of Peroxidase and its physiological significance under Karnal Bunt (KB) were determined in resistant (HD-29) and susceptible genotype (WH-542) of wheat during different developmental stages. The enzymes were expressed constitutively in both the susceptible and resistant genotype. In gel assay and differential expression analysis of POD was significantly higher (p >0.05) in Sv and S2, than the S1 and S3 stages. in silico analysis of Peroxidase for eg. physico-chemical properties, secondary structural features and phylogenetic classification for comparative analysis. Motif and Domain analysis of Peroxidase by MEME, to be important for the biological functions, and studies of evolution. Our results clearly indicate that the enhanced expression of POD at the WS2 stage, which reinforces its role in stage dependent immunity against Karnal bunt and role of POD metabolism provides genotype and stage dependant structural barrier resistance in wheat against KB.
Highlights
To investigate the role of Peroxidase and its physiological significance under Karnal Bunt (KB) were determined in resistant (HD29) and susceptible genotype (WH-542) of wheat during different developmental stages
In gel analysis of POD In order to ascertain the role of basal expression level of enzyme POD was analyzed using in gel assay at different physiological stages of wheat spikes of resistance and susceptible genotypes
Differential expression analysis of POD was carried out by using Gel documentation system, POD activity was somewhat increased significantly dominant in resistant genotype than susceptible
Summary
Protein sequences of POD from wheat, and different plants were retrieved from protein database of NCBI Ncbi.nlm.nih.gov/protein/) in FASTA format Table 1 (see supplementary material). The homology search of the POD protein was done through BLAST search tool of NCBI http:. Secondary structural properties of the POD were computed by using SOPMA
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