Abstract
Chickpea, a temperate crop, is the world’s third most important pulse crop and India produces 75% of world’s supply. The two most common types of Chickpea are the white-seeded Kabuli and the Desi variety. In vitro regeneration was achieved using cotyledonary nodal explants using desi variety seeds of cultivar K850 of Cicer arietinum . The multiple shoot induction was observed with various concentrations of auxins and cytokinins. The maximum proliferation was found at 0.5 mg/L 6-benzylaminopurine (BAP). The effectiveness was observed at various combinations. Maximum efficiency of multiple shooting was obtained at 0.5 mg/L BAP and 0.05 mg/L 2,4-dichlorophenoxy acetic acid (2,4D). For root induction, well developed shoots were transferred into rooting medium supplemented with indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA); maximum response was found at concentrations 0.10 and 0.50 mg/L, respectively. Successful rooting was recorded on media supplemented with 0.1 mg/L IBA. The well rooted plants were transferred into the soil. Key words: Explants, multiple shooting, phytohormones and acclimatization. Abbreviation: BAP , 6- Benzylaminopurine; KIN, kinetin; 2,4-D, 2,4-dichlorophenoxy acetic acid; NAA, 1-naphthaleneacetic acid; IBA, indole-3-butyric acid; IAA, indole-3-acetic acid
Highlights
Chickpea is a temperate crop, which probably originated from south-eastern Turkey and has spread to other parts of the world
The surface sterilized explants were cultured on MS medium supplemented with BAP, KIN, 2, 4-D and 1-naphthaleneacetic acid (NAA) each at five concentrations (0.05, 0.10, 0.50, 1.0 and 1.5) in individual as well as in combinations
Multiple shoots were induced from the explants after four weeks of culture on MS medium supplemented with different concentrations of BAP at
Summary
The two most common types of Chickpea are the white-seeded "Kabuli" and the "Desi "variety. In vitro regeneration was achieved using cotyledonary nodal explants using desi variety seeds of cultivar K850 of Cicer arietinum. The multiple shoot induction was observed with various concentrations of auxins and cytokinins. The maximum proliferation was found at 0.5 mg/L 6benzylaminopurine (BAP). Maximum efficiency of multiple shooting was obtained at 0.5 mg/L BAP and 0.05 mg/L 2,4-dichlorophenoxy acetic acid (2,4D). Well developed shoots were transferred into rooting medium supplemented with indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA); maximum response was found at concentrations 0.10 and 0.50 mg/L, respectively. Successful rooting was recorded on media supplemented with 0.1 mg/L IBA. The well rooted plants were transferred into the soil
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