Abstract
Methods for separation of chlorogenic acid (CA) from tobacco by-products were established. The liquid chromatography tandem mass spectrometry (LC-MS) method for identification and analysis of chlorogenic acid from tobacco materials has been developed. CA was isolated by polyamide and further purified by silicagel column chromatography. Results reveal that polyamide is suitable for separation of CA from tobacco extract. After one run treatment with polyamide, the content of chlorogenic acids was 40.3%. The product was further purified using silicagel column chromatography; the content of total chlorogenic acid was increased 2.29-fold from 40.3 to 92.2%. The LC-MS results showed that total chlorogenic acids were made up of four components: 5-CQA, 3-CQA, 4-CQA and cis-5-CQA. The content of 5-CQA was the most (78.2%) and other three chlorogenic acid derivatives were 1.9, 10.1 and 2.0%, respectively. Key words : Tobacco by-products, chlorogenic acid, polyamide, silica gel column, LC-MS.
Highlights
Tobacco, an herbaceous plant, is one of the important economic crops in the world
The liquid chromatography tandem mass spectrometry (LC-MS) results showed that total chlorogenic acids were made up of four components: 5-CQA, 3-CQA, 4-CQA and cis-5-CQA
We investigate the adsorption and desorption properties of chlorogenic acids on polyamide and developed an efficient method for the preparative separation of chlorogenic acids from tobacco byproducts
Summary
An herbaceous plant, is one of the important economic crops in the world. The leaves of tobacco are the most essential material for cigarette production (Chen et al, 2013); but, more than 20% of the tobacco are discarded during production, and not used for other purposes (Zhang et al, 2012). Chlorogenic acid, which is formed by esterification of caffeic acid and quinic acid, is the major phenolic compound in tobacco by-products. It is possessed of physiological effects such as antibacterial, antivirus and antioxidant It is feasible to utilize tobacco by-products as the material for separating chlorogenic acids. For example polyamide, macroporous resins (Wan et al, 2014; Zhang et al, 2008), silica gel column are used in separating and purifying chlorogenic acid. Chlorogenic acids in the final products were identified by liquid chromatography tandem mass spectrometry
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