Abstract
Sorghum (Sorghum bicolor (L.) Moench) is an important staple food for about 500 million people in semi arid regions of the world. Recently, sorghum has been identified as a main plant species for the comparative analysis of grass genomes and as a source of beneficial genes for agriculture. Recent studies have shown that there is conservation of gene order at the chromosome level in rice, sorghum and maize. Therefore, a high-resolution alignment between these genomes will be needed to utilize them constructively for sorghum gene discovery. Sorghum sucrose synthase gene fragment was amplified by primers designed at conserved exon position of cereal sucrose synthases. Sorghum sucrose synthase gene fragment I shared homology with other cereal sucrose synthase at the exon positions 6, 7, 8, 9 and 10. Sorghum sucrose synthase fragment II shared homology from exon 2 to 6. Key words: Sorghum, sucrose synthase, multiple sequence alignment.
Highlights
Sorghum, a C4 grass that diverged from maize just 15 million years ago, is the fifth most important cereal grown worldwide (Doggett, 1988)
This study reports on multiple sequence alignment of sucrose synthase gene sequences from different cereals and isolation and sequencing of sucrose synthase gene fragment from sorghum using primers designed at their conserved exons
Various cereal sucrose synthase gene sequences taken for MSA are summarized in the table with their gene name, accession number, exon numbers, exon position and region of homology (Table 2)
Summary
A C4 grass that diverged from maize just 15 million years ago, is the fifth most important cereal grown worldwide (Doggett, 1988). Sorghum has been identified as a key plant species for the comparative analysis of grass genomes and as a source of beneficial genes for agriculture. Sorghum is relatively small genome (750 Mbp) (Arumuganathan and Earle, 1991)) and incremental divergence from maize and rice (Doebley et al, 1990), make it ideally suited to aid the discovery and analysis of grass genes through comparative genomics. This study reports on multiple sequence alignment of sucrose synthase gene sequences from different cereals and isolation and sequencing of sucrose synthase gene fragment from sorghum using primers designed at their conserved exons
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