Abstract
Phytolacca dodecandra L’Heit (Endod var. E-44) is a medicinal plant of high saponin content used as a snail control agent in schitosomiasis. The aim of this study was to develop a micrpropagation protocol for P. dodecandra . Nodal and shoot tip explants were sterilized and cultured on MS medium supplemented with different types of concentrations and combinations of auxin and cytokinin for shoot induction, multiplication and root formation. Sodium hypochlorite at a concentration of 1.56% exposure of 12 min gave high percentages (81.67%) of survived explants for nodal followed by 1.3% concentration of NaOCl exposure of 10 min (73%) for shoot tip. 6-Benzylaminopurine (2.22 μM) was found to be an optimum concentration for shoot induction, yielding 80% for nodal and 70% for shoot tip explants. The combination of BAP (2.22 μM) with kinetin (2.33 μM) was obtained as optimum concentration yielding 13.4 and 11.03 shoots per explants for nodal and shoot tip, respectively for shoot multiplication. Half - strength MS medium with IBA (2.45 μM) and IAA (2.85 μM) yielded more than 90% root formation with optimum root number and length. Sterilized soil mix of 2:1:1 (top forest soil: coffee husk: sand) was optimized yielding 80% on transparent polyethylene plastic box. This developed micropopagation protocol is highly significant for large scale propagation of this species as a source of saponin extraction for drug development against endemic schitosomiasis. Key words: Explants, E-44 cultivar, plant growth regulators, micropropagation, detergents.
Highlights
4) is a medic cinal plant off high sapon nin content u used as a snail contro ol agent in schitosomias s sis
The present study revealed highly significant effect between treatments (BAP*kinetin), between treatments and explants for shoot multiplication and elongation, whereas there was no significant effect among explants for shoot length
Concentrated sterilants give free of microbial contamination but less number of survived plantlets due to synergetic effect to the plant tissue
Summary
4) is a medic cinal plant off high sapon nin content u used as a snail contro ol agent in schitosomias s sis. Nodal and shoot s tip ex xplants werre sterilized and culturred on MS medium supplementted with diffe erent types of o concentra ations and co ombinations s of auxin an nd cytokinin for shoot induction, multiplicatio m n and root fo ormation. 6-Benzy ylaminopurin ne (2.22 μM)) was found to be an optimum co oncentration n for shoot in nduction, yie elding 80% fo or nodal and d 70% for sh hoot tip expla ants. Sterilized so oil mix of 2::1:1 (top forrest soil: cofffee husk: sa and) was optimized yielding y. This develop ped micropo opagation protocol is s highly significant for large scale propagatio on of this species as a source off saponin extraction for f drug deve elopment against endem mic schitosom miasis
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