Abstract
Paecilomyces lilacinus is known as an effective parasite on nematodes which cause diseases to plants. P. lilacinus shows potential as a biocontrol agent against plant parasitic nematodes. The objective of this study is to optimize culture methods including nutritional requirements and environmental factors. The optimized culture conditions for biomass yields of P. lilacinus M-14 were spore suspension on basal medium (sucrose 19.00 g, soy peptone 4.06 g, K2HPO4 1.00 g, KCl 0.50 g, MgSO4 0.50 g, FeSO4 0.01 g and 17.00 g Bactor) for the first stage culture of 4 days under room condition for fungal growth, and then moved to another medium (maltose 5.00 g, soy peptone 2.50 g, ZnSO4·7H2O 0.25 gL-1, Na2MoO4·2H2O 0.005 gL-1, H3BO4 0.005 gL-1, CuSO4·5H2O 0.01 gL-1 and 17.00 g Bactor) for another 4 days culture. The environmental factors combination was water potential -1.2 MPa/pH 3/light 12 h/temperature 29°C for biomass yields, and for sporulation of P. lilacinus M-14 under the environmental conditions, it was water potential -1.2 MPa/pH 3/24 h light/29°C. It will provide valuable insight into culturing of the biocontrol fungus. Key words: Biomass, environment, Paecilomyces lilacinu, biocontrol fungus.
Highlights
With the increase in awareness of the harmful effects of chemical pesticides and the changing public attitude towards environmental pollution, chemical pesticides is losing their popularity among farmers (Pandey et al., 2000; Anastasiadis et al, 2008)
The aim of this study is to optimize the biomass yields of P. lilacinus based on its growth on a broad culture medium, such as PDA, PDB, PCA, Czapek and which have better growth and sporulation on Czapek, after 8
After the nutrition combination by full experiment, the combination of nutrition together with environmental factors was optimized for sporulation of P. lilacinus M-14 by L16(215)
Summary
With the increase in awareness of the harmful effects of chemical pesticides and the changing public attitude towards environmental pollution, chemical pesticides is losing their popularity among farmers (Pandey et al., 2000; Anastasiadis et al, 2008). Environmental concerns for the quality of the environment and food safety have created social and legislative pressure to remove many agricultural pesticides from the market (Noling and Dickson, 1992; McKenry et al, 1994). Biological control is considered as the most safe and effective alternative tochemical control methods (Kutschera and Hossfeld, 2012; Sharma et al, 2014; Liu et al, 2017).
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