Abstract
Infectious bronchitis (IB) is one of the devastating chicken viral diseases that causes significant financial loses to chicken industry worldwide. In this study, highly purified recombinant nucleocapsid protein (rNP) of IB virus (IBV) was used to develop an in-house enzyme linked immunosorbent assay (iELISA) for the detection of IB. The iELISA was optimized to achieve maximum sensitivity and specificity. All tested broiler chicken farms were positive for IBV antibody in a commercial ELISA kit (cELISA) and the optimized iELISA. Thirty three (33) specific pathogen free (SPF) chickens and 20 broiler chickens held in a restricted environment tested negative for IBV antibody in both ELISA. The above results suggest that the iELISA is a sensitive and specific test for the detection of antibody to IBV in chicken flocks. Key words: Enzyme linked immunosorbent assay (ELISA), infectious bronchitis (IB), recombinant nucleocapsid protein (rNP).
Highlights
Infectious bronchitis (IB), a highly contagious upper respiratory disease, was first reported in 1931 (Schalk and Hawn, 1931; Collisson et al, 1992; Cavanagh and Naqi, 2003) and continues to be one the major threats to chicken industry worldwide
All tested broiler chicken farms were positive for IB virus (IBV) antibody in a commercial Enzyme linked immunosorbent assay (ELISA) kit and the optimized in-house enzyme linked immunosorbent assay (iELISA)
Thirty three (33) specific pathogen free (SPF) chickens and 20 broiler chickens held in a restricted environment tested negative for IBV antibody in both ELISA
Summary
Infectious bronchitis (IB), a highly contagious upper respiratory disease, was first reported in 1931 (Schalk and Hawn, 1931; Collisson et al, 1992; Cavanagh and Naqi, 2003) and continues to be one the major threats to chicken industry worldwide. The disease has been described in young and laying chickens with age-dependent clinical signs. Common respiratory signs including tracheal rales, sneezing and coughing are observed. IBV-induced clinical signs in adult laying chickens are poor egg quality and significant reduction in egg production (Ignjatovic and Sapats, 2000; Hofstad, 1984). Many serotypes have been reported for IBV (Cavanagh, 2007; Ignjatovic and Sapats, 2000). Partial NP has been utilized for the development of an IBV ELISA (Lugovskaya et al, 2006)
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