Abstract
Clinacanthus nutans is an economically important medicinal plant that can be found grown in many countries in the Asian region. Useful medicine properties such as anti-cancer, anti-bacteria, and anti-viral, backed by its high content of phytochemical compounds such vitexin, isovitexin, stigmasterol and lupeol has increased the demand for C. nutans in the market. Extensive work had been carried out on its content and pharmacological activity using fresh samples but limited studies for in vitro cultures of C. nutans. No genome size estimation has been done for C. nutans. Objective of this study was to analyse nuclear DNA content of C. nutans using flow cytometry. Preparation of different nuclei isolation buffer and stoichiometric DNA staining using propidium iodide was carried out. The genome size of C. nutans was estimated using Glycine max cv. Polanka as internal standard and its genome size was compared with in vitro plantlets of C. nutans. Flow cytometry analysis revealed that nuclear 2C DNA of C. nutans content is estimated at 1.75 ± 0.006 pg. Coefficient of variation in flow cytometric analysis was within the limit of 5% implying that the results were reliable with the Tris.MgCl2 being the best nuclei isolation buffer. No significant difference was observed from field grown and in vitro C. nutans. This finding will assist further in genome size evolution analysis of Clinacanthus spp. and to determine polyploids for increased active compounds and biomass. Key word: Flow cytometry; Nuclear DNA content, Clinacanthus nutans.
Highlights
Clinacanthus nutans, known as Sabah Snake Grass or Daun Belalai Gajah is a shrub belonging to the Acanthaceae family
The choice of nuclei isolation buffer is a crucial step in genome size estimation in plants
Three different buffers were examined here for C. nutans as these three buffers (Tris.MgCl2, LBO1 and Otto buffer) differ in their ingredients to extract the nuclei from the plant
Summary
Clinacanthus nutans, known as Sabah Snake Grass or Daun Belalai Gajah is a shrub belonging to the Acanthaceae family. This plant has recently gained much economic value in South Asian region mainly in Malaysia, Indonesia, and Thailand due to its pharmacological properties (Zulkipli et al, 2017). Based on the active compounds, pharmacological activities such as anti-oxidative (Pannangpetch et al, 2007; Arullappan et al, 2014), anti-proliferative (Yong et al, 2013; Ghazemzadeh et al, 2014), anti-tumorigenic (Huang et al, 2015), anti- bacterial (Chomnawang et al, 2009; Arullappan et al, 2014), anti-viral (Kunsorn et al, 2013) and anti-inflammatory (Wanikiat et al, 2008) have been reported with C. nutans.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
