Abstract
Citrus nobilis Lour. Gonggan is an excellent fruit variety which is widely planted in South China. The origin of Gonggan is not clear. It is conjectured that its origin is from a cross between tangerine and orange; however, there is no direct evidence to confirm this. Here, we applied the optimized random amplified polymorphic DNA (RAPD)-PCR to amplify genus Citrus species: 1) to better understand the genetic relationship between C. nobilis Lour. Gonggan and other Citrus species; and (2) to address the phylogenetic relationship among Citrus species. A total of 21 RAPD primers were used to screen 4 Citrus species and 10 of them efficiently amplified the genomic DNA of 23 Citrus accessions. A total of 87 locus/alleles were generated by those 10 primers with an average of 97.7% polymorphic. Our data supported that C. nobilis Lour. Gonggan belongs to a big group with most tested tangerine and orange and a subgroup with Citrus haniana and Citrus flamea, implying that either C. haniana or C. flamea is likely to be one of the parents of C. nobilis Lour. Gonggan.
Highlights
A total of 21 Random amplified polymorphic DNA (RAPD) primers were used to screen 4 Citrus species and 10 of them efficiently amplified the genomic DNA of 23 Citrus accessions
Gonggan belongs to a big group with most tested tangerine and orange and a subgroup with Citrus haniana and Citrus flamea, implying that either C. haniana or C. flamea is likely to be one of the parents of C. nobilis Lour
Our results show the phylogenic relationship of Citrus cultivars and potential patents of C. nobilis Lour
Summary
Sihui County is the origin of Gonggan in China. Based on the Sihui county records, Gonggan is the natural hybrid of tangerine and orange, but its exact parents is not recorded and as a variety it retained after several generation’s selection. The local citrus varieties include C. flamea Hort. Ex Tseng bayueju and C. flamea Hort. Most likely has some relationship with some of the historical planted cultivars. RAPD technique was applied to identify the origin and phylogenetic relationship of Gonggan. Twenty three Citrus cultivars (2 accessions) and RAPD-PCR reactions were conducted. Our results show the phylogenic relationship of Citrus cultivars and potential patents of C. nobilis Lour.
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