Abstract
The taxonomic position of an actinomycete isolated from a soil sample collected from Wadi Sannur in Egypt was established using a polyphasic approach. The isolate, which was designated WS 51T, was shown to have chemical and morphological properties typical of streptomycetes. An almost complete 16S rDNAgene sequence of the strain was generated and compared with corresponding sequences of representative streptomycetes. The resultant data confirmed the classification of the strain in the genusStreptomyces but also showed that it formed a distinct phyletic line within the 16S rDNAStreptomyces gene tree. The organism was most closely associated to the type strains of Streptomyces hygroscopicus, Streptomyces malaysiensis and Streptomyces yatensis but was readily separated from them using a range of phenotypic properties. It is proposed that strain WS 51T (= CCTCC 001032T = DSM 41834T) be classified in the genus Streptomyces as Streptomyces sannurensis sp. nov. Key words: Streptomyces sannurensis sp. nov., polyphasic taxonomy, alkaliphilic streptomycete.
Highlights
The genus Streptomyces encompasses aerobic, Grampositive actinomycetes that form an extensively branched, substrate mycelium, aerial hyphae which typically differentiate into chains of spores, have LLdiaminopimelic acid but lack major characteristic sugars in whole-organism hydrolysates and contain DNA rich in guanine plus cytosine (Williams et al, 1989; Manfio et al, 1995)
The organism was most closely associated to the type strains of Streptomyces hygroscopicus, Streptomyces malaysiensis and Streptomyces yatensis but was readily separated from them using a range of phenotypic properties
In the course of a screening programme designed to isolate novel bioactive actinomycetes from environmental samples collected from Wadi Sannur in the Eastern Desert of Egypt, an actinomycete, designated WS 51, was isolated and provisionally assigned to the genus Streptomyces
Summary
The genus Streptomyces encompasses aerobic, Grampositive actinomycetes that form an extensively branched, substrate mycelium, aerial hyphae which typically differentiate into chains of spores, have LLdiaminopimelic acid but lack major characteristic sugars in whole-organism hydrolysates (wall chemotype 1 sensu: Lechevalier and Lechevalier, 1970) and contain DNA rich in guanine plus cytosine (Williams et al, 1989; Manfio et al, 1995). The taxon contains more than 600 validly described species, representatives of which have been assigned to lumpy groups based on 16S rDNAsequence data (Bull et al, 2005). It is evident that the genus Streptomyces remains underspeciated (Sembiring et al, 2000; Manfio et al, 2003) though several validly. Novel streptomycetes are still in great demand as a source of new commercially significant bioactive compounds (Bérdy, 1995; Holtzel et al, 2003; Xu et al, 2004). In the course of a screening programme designed to isolate novel bioactive actinomycetes from environmental samples collected from Wadi Sannur in the Eastern Desert of Egypt, an actinomycete, designated WS 51, was isolated and provisionally assigned to the genus Streptomyces.
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