Abstract
Q fever, caused by Coxiella burnetti is a well recognised zoonotic disease around the world which is endemic in many countries. In Bangladesh, a very few information is available in men and animals about this disease. Thus, the objective of this study is to determine the presence of Q fever in animal (goat and cattle) and human population including the tick which is a vector of the disease. A total of 172 animals with abortion and reproductive disorder history were collected from Shahjadpur Upazilla of Sirajgonj district in Bangladesh. 150 sera samples from patients of Mymensingh Medical College Hospital were collected which had a history of pyrexia. 127 tick samples were collected from the body of animals randomly. The samples were tested with CHEKIT Q-Fever antibody Enzyme Linked Immunosorbent Assay (ELISA) Test Kit (indirect ELISA) primarily and the positive sere were retested by real time polymerase chain reaction (PCR) to detect DNA of C. burnetii. Indirect ELISA revealed the prevalence result of 6.97 and 0% in animals and humans, respectively and only one tick was Reverse transcription Polymerase Chain Reaction (RT-PCR) positive (0.79%). It is thus observed that this is the first time the presence of C. burnetii in ticks using real time PCR is reported. Key words: Coxiella burnetti, Enzyme Linked Immunosorbent Assay (ELISA) antibody, polymerase chain reaction (PCR), sera samples.
Highlights
Q fever is a disease which is zoonotic in nature caused by Coxiella burnetii
The samples were tested with CHEKIT Q-Fever antibody Enzyme Linked Immunosorbent Assay (ELISA) Test Kit primarily and the positive sere were retested by real time polymerase chain reaction (PCR) to detect DNA of C. burnetii
The overall seropositivity of C. burnetii antibodies were 6.97% in animal and 0% in humans and no positive seropositive sera was found by real time PCR (Table 1)
Summary
Human infections with C. burnetii are usually transmitted by aerosol during contact with infected animal feces or bird residues (Tissot-Dupont et al, 2004). Significant economic losses occurred when a farm is infected with Q fever (Porter et al, 2011). In humans, it affects the respiratory system, musculoskeletal system, digestive system, nervous system and cardiovascular system (Karakousis et al, 2006)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
