Abstract
This study sought to evaluate the behavioural and biochemical effects of Dextran Sodium Sulphate (DSS) on the oxidative stress biomarkers and antioxidant enzymes. Drosophila melanogaster (both sexes) of 3 days old were exposed to various DSS incorporated diets at concentrations, 0.5 - 3.0% for a period of 14 days (Survival rate). The second phase comprised of 3 groups; Group I- Control (normal diet), Group II-0.5% DSS, and Group III – 1.0% DSS and fed for 5 days. Climbing activity and biochemical assays were then determined. The survival rates of the flies with concentrations above 1.0% were highly reduced. The induced oxidative stress caused by DSS showed an impaired climbing activity, a significant (p<0.05) increase in the catalase enzymatic activity and malondialdehyde content in both Groups II and III in relation to the flies in Group I (control). Also there was significant (p<0.05) inhibition of GST activity and reduction of total thiol contents in group III in comparison to the control. In conclusion, the DSS dose- dependent toxicity effect was revealed by the increase in the malondialdehyde contents and catalase enzymatic activity. Key words: Drosophila melanogaster, oxidative stress, pro-oxidant, antioxidant enzymes, dextran sodium sulphate.
Highlights
Drosophila melanogaster is a dipteran insect with two wings; typical characteristic of the “true flies”
Dextran sodium sulphate (DSS) has been used over a period of time to induce inflammation- pro-oxidative mechanisms (Amcheslavsky et al, 2009; Jianming et al, 2010; Mittal et al, 2014)
Incorporation of varying concentration of DSS into the diet was used for the first phase, to investigate if its pro-oxidant effect can induce oxidative stress using Drosophila melanogaster as a model
Summary
Drosophila melanogaster is a dipteran insect with two wings; typical characteristic of the “true flies”. Since one hundred years ago, these flies have been introduced as a research model in the studies of genetics and other related aspects of molecular biology. Drosophotoxicology has been introduced, which is a toxicology study in which parts or whole of the fly is used (Rand, 2010; Chifiriuc et al, 2016). It meets the standard of the European Centre for the Validation of Alternative
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