Abstract
Indolocarbazole (ICZ) alkaloid constitutes a group of natural products which manifested powerful biological activity, especially anticancer. However, it was laborious to find ICZs-yield organism when anticancer activity was adopted as selection target. The specificity of 6 primer pairs was detected and the results revealed that primer rebBN1/rebBC1 possessed preferable specificity because indolocarbazole-producers DNA was exclusively amplified and no target fragment was amplified from any of indolocarbazole nonproducers. Therefore, primer rebBN1/rebBC1 could specifically identify indolocarbazole-producers from microorganism strains. Key words: Indolocarbazole, rebBN1/rebBC1, ICZs-producing microorganism screening.  
Highlights
The indolocarbazole (ICZ) family is an important class of natural products isolated from bacteria, actinomycetes, cyanobacteria, fungi, slime moulds and marine invertebrates (Sanchez et al, 2006)
Indolocarbazole (ICZs) compounds are structurally characterized by possessing an indolo[2,3a]pyrrolo[3,4-c ]carbazole core derived from two units of tryptophan, with sugars attached derived from glucose and methionine
Different open reading frames (ORFs) fragments were amplified using specific primers and the results showed that for almost primers except StaPH/StaPN visible fragments were amplified only when chromosomal DNAs from indolocarbazole producers were used as templates
Summary
The indolocarbazole (ICZ) family is an important class of natural products isolated from bacteria, actinomycetes, cyanobacteria, fungi, slime moulds and marine invertebrates (Sanchez et al, 2006). Indolocarbazole (ICZs) compounds are structurally characterized by possessing an indolo[2,3a]pyrrolo[3,4-c ]carbazole core derived from two units of tryptophan, with sugars attached derived from glucose and methionine. According to their structural features and mechanisms of actions, ICZs are divided into two major classes (Gribble and Berthel, 2001). The first class was characterized by a sugar moiety linked to both indole nitrogen atoms of the indolocarbozole core to form a. The most attracting point is their therapeutic potential as anti-cancer drugs. Their strong effects to various harmful insects were discovered by our team (related article under review). In order to solve the problem, the feasibility of using molecular probes combined with HPLC to detect ICZs-producing microorganisms was investigated in this article
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