English

Jericoacute Bello Bello,Iglesias Iglesias,Nancy Santana Buzzy,Laacute Zaro Saacute Nchez Velaacute Squez,Lourdes Reu,Joseacute Casas Martiacute Nez

doi:10.5897/ajb12.2180

Abstract

This paper describes two in vitro regeneration systems through direct and indirect organogenesis in Pinus brutia using fascicles aseptic cultures as explants. Mechanical scarification and gibberellic acid (GA 3 ) were evaluated on in vitro seed germination. Scarification was the treatment that allowed for in vitro seed germination. The highest direct organogenic response was obtained in Murashige and Skoog (MS) medium containing 4.5 µM thidiazuron, whereas the highest indirect organogenesis was obtained with 9.8 µM thidiazuron and 3.4 µM paclobutrazol. The isolated shoots were rooted on MS medium supplemented with 1.70 µM indoleacetic acid. A large variation in root ability was observed among plantlets. These results suggest that both regeneration systems can be applied to the micropropagation or genetic transformation of P. brutia . Key words : Pinus brutia , organogenesis, fascicles, paclobutrazol, thidiazuron.

Highlights

IntroductionPinus brutia Ten. var. eldarica (Medw.) (commonly known as Calabrician pine) is a native and restricted species in the eastern Mediterranean
Pinus brutia Ten. var. eldarica (Medw.) is a native and restricted species in the eastern Mediterranean
Dry seeds of P. brutia Ten. var. eldarica (Medw.) were obtained from a germplasm seed bank located in Murcia, Spain, and were used to produce the aseptic seedlings that were the source of the explants

Summary

Introduction

Pinus brutia Ten. var. eldarica (Medw.) (commonly known as Calabrician pine) is a native and restricted species in the eastern Mediterranean. And economically speaking, it is one of the most important forest tree species in this region (Boydak, 2004). The production of rooted cuttings is very poor because of the high degree of lignification of the branches and the low rooting capacity of cuttings (Orea and Villalabos, 1990). In vitro regeneration can be very effective in the micropropagation and/or genetic breeding of several commercially important forest trees. Regeneration through morphogenesis has been extensively described in a number of forest micropropagation protocols in different species of the genus Pinus: P. taeda L. (Tang et al, 1998), P. wallichiana AB Jacks (Mathur and Nadgauda, 1999), P. taeda L. (Tang, 2001), P. taeda L. Regeneration through morphogenesis has been extensively described in a number of forest micropropagation protocols in different species of the genus Pinus: P. taeda L. (Tang et al, 1998), P. wallichiana AB Jacks (Mathur and Nadgauda, 1999), P. taeda L. (Tang, 2001), P. taeda L. (Tang and Guo, 2001), P. heldreichii (Stojičić and Budimir, 2004), P. pinea L. (Sul and Korban, 2004), P. virginiana (Tag et al, 2004), P. strobus L. (Tang and Newton, 2005), P. elliottii (Tang et al, 2006), P. sylvestris L. (De Diego et al, 2010) and P. radiata (Montalbán et al, 2011)

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