English

Suneja Pooja,Dahiya Pushpa,Kumar Ajit

doi:10.5897/ajmr12.2192

Abstract

Twenty eight isolates associated with the nodule surface of two chickpea cultivars, that is, reverted non-nodulating (ICC4993NN(R)) and normal chickpea (HC5) cultivars were isolated. All of them were found to be Gram positive. The isolates NE81, NE82 from cv ICC4993NN(R) and NE1043 from cv HC5 inhibited the growth of isolates NE84, NE44, NE45, NE32, NE16-1, NE32, NE84, NE44, NE19, NE26 and NE14, NE53, NE16-2, NE18, NE1043, respectively. However, some of these nodule epiphytic bacteria promoted the growth of mesorhizobial isolates from chickpea. The co-inoculation of these nodule epiphytes with mesorhizobial isolate resulted in significant increase in nodulation and shoot dry weight in chickpea as compared to the inoculation only with nodule epiphytes.   Key words: Chickpea, nodules, epiphyte, growth inhibition.

Highlights

Rhizosphere is microbe and nutrient rich environment where the roots exude water and other compounds, thereby providing favourable environment for the growth of microorganisms
The nodules when, mildly surface sterilized with 0.1% HgCl2 and placed on Tryptone Soya Agar (TSA) plates showed growth around these partially sterilized nodules (Figure1a)
28 Gram positive nodule epiphytes were obtained from both cultivars (Table 1)

Summary

Introduction

Rhizosphere is microbe and nutrient rich environment where the roots exude water and other compounds, thereby providing favourable environment for the growth of microorganisms. Rhizospheric microorganisms have different types of interactions with plant roots and nodules in the rhizosphere (Pinton et al, 2001; Werner, 2004; Hayat et al, 2010). These plant-microbe interactions influence the plant growth. Bacteria are predominantly found in the root rhizosphere and are commonly termed rhizobacteria (Antoun and Kloepper, 2001) but can be referred to as root epiphytes These root epiphytes can have beneficial, damaging or harmless effects on plant growth. A total of 150 bacterial isolates belonging to Bacillus, Pseudomonas, Azotobacter and Rhizobium were reported from different rhizospheric soil of chick pea in the vicinity of Allahabad, India (Joseph et al, 2007)

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