Abstract
The objective of this study was to detect dairy cattle infected with Brucella abortus from Jamalpur, Rangpur districts and Central Cattle Breeding and Dairy Farm (CCBDF), Savar, Dhaka in Bangladesh. Both milk and serum samples of 510 dairy cattle were initially screened by milk ring test (MRT) and Rose Bengal Test (RBT). Twelve samples positive in both MRT and RBT were further confirmed by conventional polymerase chain reaction (PCR). The overall prevalence of brucellosis based on MRT and RBT was 2.7 and 2.4%, respectively. The prevalence of brucellosis was found to be significantly (p<0.001) higher in CCBDF than Jamalpur district. Out of 7 MRT and RBT positive samples, 42.9% samples of CCBDF were PCR positive and none of the five samples of Jamalpur and Rangpur districts was PCR positive. These results strongly suggest that the use of MRT, RBT and PCR technique could lead to more reliable diagnosis of brucellosis from dairy cow in Bangladesh. Key words: Seroprevalence, molecular detection, brucellosis, dairy cattle, Bangladesh.
Highlights
Brucellosis is an ancient and one of the world’s most widespread zoonotic diseases affecting both, public health and livestock production (Ariza et al, 2007), which is caused by Gram-negative, facultative intracellular bacteria of the genus Brucella
Out of 7 milk ring test (MRT) and Rose Bengal Test (RBT) positive samples, 42.9% samples of Central Cattle Breeding and Dairy Farm (CCBDF) were polymerase chain reaction (PCR) positive and none of the five samples of Jamalpur and Rangpur districts was PCR positive. These results strongly suggest that the use of MRT, RBT and PCR technique could lead to more reliable diagnosis of brucellosis from dairy cow in Bangladesh
The overall seroprevalence of bovine brucellosis reported in Bangladesh is 5.3% (4.8 to 6.2) (Ahmed et al, 1992; Amin et al, 2004, 2005; Nahar and Ahmed, 2009; Ahasan and Song, 2010; Rahman et al, 2012; Sikder et al, 2012; Belal and Ansari, 2013; Dey et al, 2013; Islam et al, 2013) and overall prevalence of brucellosis in cows based on milk ring test (MRT) is 5.6% (4.8 to 6.3) (Pharo et al, 1981; Rahman and Rahman, 1981; Rahman et al, 1983; Sikder et al, 2012)
Summary
Brucellosis is an ancient and one of the world’s most widespread zoonotic diseases affecting both, public health and livestock production (Ariza et al, 2007), which is caused by Gram-negative, facultative intracellular bacteria of the genus Brucella. B. abortus DNA has been detected from bovine milk and serum samples using real time polymerase chain reaction (PCR) assays (Rahman et al, 2014, 2017) in Bangladesh. Zoonotic transmission occurs most frequently via unpasteurized milk products in urban settings, while occupational exposure of farmers, veterinarians, or laboratory workers can result from direct contact with infected animals or tissues or fluids associated with abortion (Olsen and Palmer, 2014). Long time direct contact with infected cows may be responsible for such a higher level of brucellosis among dairy workers as consumption of raw milk is unusual in Bangladesh. This study describes the Rose Bengal, MRT and conventional PCR based detection of B. abortus infected dairy cattle in Bangladesh
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