Abstract
Microrhizomes were produced from leaf disc derived callus of a threatened endemic medicinal plant - Decalepis hamiltonii. Murashige and Skoog (MS) medium supplemented with 2 mM 6-benzyleaminopurine (BAP) and 6 mM 1-napthaleneacetic (NAA) acid was found to be optimum for rapid callus induction and establishment from leaf disc explants. Further differentiation of callus into microrhizome was conquered in MS medium supplemented with 4 mM indole-3-butyric acid (IBA) and 8 mM NAA. A maximum of 20 microrhizomes in a cluster was produced within 90 days. Yeast extract (0.05%) and polyvinylpyrrolidone (0.05%) further enhanced the microrhizome formation when supplemented along with plant growth regulators (PGRs). Key words: Decalepis hamiltonii, microrhizome, herbal medicine, secondary metabolites.
Highlights
There has been a gradual revival of interest in the use of medicinal plants in developed as well as developing countries since herbal medicines have been reported to be safe without side effects (Bahadur et al, 2007)
Among different plant growth regulators (PGRs) combinations used, Murashige and Skoog (MS) medium supplemented with NAA (6 μM) along with BAP (2 μM)
The partially developed microrhizome clusters were transferred to solid medium where they grow faster and produced higher number of microrhizomes (Table 2)
Summary
There has been a gradual revival of interest in the use of medicinal plants in developed as well as developing countries since herbal medicines have been reported to be safe without side effects (Bahadur et al, 2007). It prefers to grow along rocky slopes, big rock boulders and rocky crevices and small mounds at an altitude from 300 to 1200 m (Ravikumar and Ved, 2000) Rhizome extract of this plant is reported to have antioxidant, antibacterial, hepatoprotective, antiinflammatory, insecticidal, antidiabetic, anti-atherosclerotic, immunomodulatory and antitumour potentials due to the presence of bioactive principles such as decalepin, salicylaldehyde, vanillin anisaldehyde, 2-hydroxy 4methoxy benzaldehyde and 5,7,4-trihydroxy flavanone 4'o-β-D-glucoside (Srivastava and Shivanandappa, 2006, 2010; Harish and Shivanandappa, 2011; Ashalatha et al., 2010; Naveen and Khanum, 2010; Sumalatha et al., 2010; Thangavel et al, 2011). A rapid reproducible protocol for microrhizome production from leaf disc derived callus of D. hamiltonii have been devised in this present study
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