Abstract
The purpose of the present investigation is to enhance production of the biomedically important enzyme, lipase, by subjecting the indigenous lipase producing fungal strain Aspergillus japonicus MTCC 1975 to strain improvement and random mutagenesis (UV irradiation, HNO2 and N-methyl-N’-nitro-N-nitroso guanidine). The isolation of mutants and the lipolytic activity of selected mutants were described. The best UV selectant (AUV3) showed 127% higher lipase activity than the parent strain. The lipase yield of the best HNO2 mutant (AHN3) was 139% higher than UV mutant (AUV3) and 177% higher than the parent strain. Also, the lipase yield of the best NTG mutant (ANT4) was 156% higher lipase activity than the HNO2 mutant (AHN3) and 217% higher than the UV mutant (AUV3) and 276% higher lipase activity than the parent strain. The results indicated that UV, HNO2 and NTG treatment were effective physical and chemical mutagenic agents for strain improvement of Aspergillus japonicus for enhanced lipase productivity. Key words: Lipase, Aspergillus japonicus, UV Irradiation, HNO2 and N-methyl-N’-nitro-N-nitroso guanidine.
Highlights
Lipases (triacyl glycerol acyl hydrolases, (E.C. 3.1.1.3) are versatile catalysts, which are used for diverse purposes by (Kajandjian et al, 1986)
The aim of the present investigation is to enhance lipase productivity of the fungal strain Aspergillus japonicus by subjecting it to improvement by random mutagenesis (UV Irradiation, HNO2 and N-methyl-N’nitro-N-nitroso guanidine, Nnitroso guanidine (NTG))
A total of 15 colonies, 5 isolates were selected from the plates showing less than 1% survival rate (120 and 150 min NTG treated spore suspension) and tested for lipase production
Summary
Lipases (triacyl glycerol acyl hydrolases, (E.C. 3.1.1.3) are versatile catalysts, which are used for diverse purposes by (Kajandjian et al, 1986). The exponential increase in the application of lipases in various fields in the last few decades demands extension in both qualitative improvement and quantitative enhancement. Strain improvement is an essential part of process development for fermentation products. Ed strains can reduce the costs with increased productivity and can possess some specialized desirable characteristics. Such improved strains can be obtained by mutation and selection depends on the alternate processes of diversification, selection and rediversification, so that better strains are successfully picked out and further improved (Rowlands, 1984). The aim of the present investigation is to enhance lipase productivity of the fungal strain Aspergillus japonicus by subjecting it to improvement by random mutagenesis (UV Irradiation, HNO2 and N-methyl-N’nitro-N-nitroso guanidine, NTG)
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