Abstract
The present study was undertaken to evaluate the most suitable concentration of growth regulators for callus induction and subsequent organogenesis in Abutilon indium (L.) Sweet (Malvaceae) leaf, stem and seed explants. Since the plant has a lot of medicinal importance, it was subjected to culture in vitro . Kinetin for seeds, 2,4-dichlorophenoxyacetic acid (2,4-D), benzyl amino purine (BAP), naphthalene acetic acid (NAA) and indole-3-acetic acid (IAA) for leaf as well as BAP and kinetin for stem were the growth regulators used at different concentrations individually. Results showed that growth regulators at different combinations on subculture resulted in the regeneration of shoots. The excised shoots were transferred to liquid medium for in vitro rhizogenesis and plantlets were acclimatized in the laboratory conditions before they were transferred to garden soil. Histologically, callus sections were with active centres, embryoids and multiple shoot buds. Cytological variations such as multinucleate, chromosome bridges, polyploidy and tracheary elements were noticed in callus cells. The regenerated plants were morphologically and cytologically similar to the in vivo plants. Key words: Embryoids, multiple shoot buds, multinucleate, polyploidy, Malvaceae.
Highlights
Abutilon indicum (Malvaceae) is a hairy medicinal undershurb with golden yellow flowers, abundant throughout the hotter parts of India
Kinetin for seeds, 2,4-dichlorophenoxyacetic acid (2,4-D), benzyl amino purine (BAP), naphthalene acetic acid (NAA) and indole-3-acetic acid (IAA) for leaf as well as BAP and kinetin for stem were the growth regulators used at different concentrations individually
The callus proliferation was observed in all the treatments and the callus on subculture at 3 mg/l benzyl amino purine (BAP) and 1 mg/l naphthalene acetic acid (NAA) and at 2 mg/l BAP and 1 mg/l NAA induced shoot buds (Figure 2) from hard green compact callus
Summary
Abutilon indicum (Malvaceae) is a hairy medicinal undershurb with golden yellow flowers, abundant throughout the hotter parts of India. Its decoction is used in bronchitis, jaundice, gonorrhoea and inflammation of the bladder It is used in mouth wash in the case of tooth ache (Kirtikar and Basu, 1918). Since this is an important medicinal plant, there is an increased demand of A. indicum biomass to meet the requirements of medicine and has generated need to undertake large scale cultivation by the donor plant. Regeneration of plants from callus may help to induce variability in this plant for future improvement. This may result in the generation of useful somaclonal variants not available by conventional methods. Whole plant regeneration from various explants of A. indicum was reported
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
