Abstract
Willow (Salix spp.) includes closely related dioecious polyploid species, which are obligate outcrossers. Natural populations of willows and their hybrids are represented by a mixture of highly heterozygous genotypes sharing a common gene pool. Random amplified polymorphism DNAs (RAPD) and microsatellites (simple sequence repeats, SSRs) are useful methods for studying genetic diversity of willow (Salix spp.). RAPD delivers a large number of data points from a single experiment and is a useful method for distinguishing between closely related individuals. SSR markers are very robust tools, and we have identified several markers that show high levels of polymorphism in willow. Genetic characterization of 94 genotypes (four female, ten male, and 80 half sibs) of Salix collected from Naganji Nursery of University of Horticulture and Forestry, Solan, Himachal Pradesh, India were analyzed using 10 SSRs and 15 RAPDs PCR-based molecular markers. RAPD analysis yielded 87 polymorphic fragments (98.9%), with an average of 5.8 polymorphic fragments per primer. Similarly, SSR analysis produced 33 bands, out of which 26 were polymorphic (78.8%) with an average of 2.6 polymorphic fragments per primer. The genetic diversity was high among the genotypes (Nei’s genetic diversity = 0.354 and Shannon’s information index = 0.536) as measured by combination of both RAPD and SSR markers. The mean coefficient of gene differentiation (Gst) was 0.037, indicating 96.6% of the genetic diversity resided within the genotypes. It was found that the genetic diversity among genotypes was broader, suggesting the importance and feasibility of introducing elite genotypes from different hybrids for willows germplasm conservation and breeding programs. Key words: Salix sp., female, male, half sibs, molecular markers, genetic diversity, Random amplified polymorphism DNAs (RAPD), simple sequence repeats (SSRs).
Highlights
Congrruence e of ran ndom ampliffication n of po olymorrphic deoxy yribonucleic acid (RAPD)) and s simple e seque ence repeats (SSR) marrkers in gene etic ch haracte erizatio on of willow w (Salix x spp.)
Genetic g of Salix co ollected from m Naganji characterization of 94 genotypes
Total genomic DNA was extracted from the frozen leaves (2 g) by the CTAB method (Saghai-Maroof et al, 1984) with minor modifications, which included the use of 200 mg of polyvinyl pyrollidone per sample
Summary
Congrruence e of ran ndom ampliffication n of po olymorrphic deoxy yribonucleic acid (RAPD)) and s simple e seque ence repeats (SSR) marrkers in gene etic ch haracte erizatio on of willow w (Salix x spp.). Manoj Kuma ar Singh1*,, Naresh Bahadur Sin ngh[1], Hari Paul Sankhyan[1] and Pradee ep Kumar N. Departmen nt of Tree Imp provement an nd Genetic Re esources, Co llege of fores try, Dr Y. S. Parmar Univversity of Horticulture and a Forestry y, Nauni, Sola an (H.P.) 173
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
