Abstract
DNA barcoding is a widely used molecular approach for species cataloging for unambiguous identification and conservation. In the present study, DNA barcoding of some West African mammals were performed with six new mitochondrial CO1 sequences for Civettictis civetta, Tadarida nigeriae, Orycteropus afer, Heliosciurus gambianus, Equus africanus asinus and Funisciurus anerythrus which are absent in public databases such as BLAST/NCBI and BOLD. Sequence identifications were made by comparing unknown sequences against the DNA barcodes of known species through distance-based tree construction and alignment probing. The sequences have been deposited to GenBank/NCBI. Key words: mtDNA, West African mammals, conservation, biodiversity.
Highlights
West Africa is endowed with rich flora, fauna and variety of ecosystems
DNA barcoding of some West African mammals were performed with six new mitochondrial CO1 sequences for Civettictis civetta, Tadarida nigeriae, Orycteropus afer, Heliosciurus gambianus, Equus africanus asinus and Funisciurus anerythrus which are absent in public databases such as BLAST/NCBI and BOLD
Development of a reference DNA barcode library of all the known species identified by taxonomists are the first and most important step to establish DNA barcoding as a tool for accurate species identification
Summary
West Africa is endowed with rich flora, fauna and variety of ecosystems. These ecosystems contain wide variety of endemic species with highly restricted ranges within the region. DNA barcoding is a widely used molecular approach for species cataloging for unambiguous identification and conservation. DNA barcoding of some West African mammals were performed with six new mitochondrial CO1 sequences for Civettictis civetta, Tadarida nigeriae, Orycteropus afer, Heliosciurus gambianus, Equus africanus asinus and Funisciurus anerythrus which are absent in public databases such as BLAST/NCBI and BOLD.
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