Abstract
Toxicity of the freeze-dried micro-aquatic planktonic cyanobacterium Oscillatoria agardhii, dominating and isolated from Makkah –KSA was studied. Microcystins were detected from freeze-dried cells using high pressure liquid chromatography HPLC. The histopathological examination of mice liver injected on week basis with diluted 1/5 of the lethal dose, (105 mg/ Kg body weight), of toxin extract revealed sever changes in liver histology and displayed apparent signs of degenerative hepatic structure. Cytoplasmic vacuolation and parenchyma exhibit hepatocytes degeneration. Hepatic vasculature and biliary system were blocked and severely damaged. The effect of the extract on blood contents and liver function of white mice was investigated. Mice were divided into three groups and injected intraperitoneally (i.p) with weekly reciprocal doses of toxin extract as 21, 42 and 63 mg/ mice, respectively, for seven weeks. Mice injected with 63 mg were the mostly affected and showed signs of acute cellular and physiological damage involving oligocythemia, leucoytosis, marked increase in serum urea, cholesterol, triglycerides, creatinine, Aspartate Aminotransferase (AST), alanine aminotransferase (ALT), hematocrit (HCT) and mean cell hemoglobin concentration (MCHC), while blood platelets showed abnormal increase which suggest an inhibitory action on haemopiesis. In addition, the abnormal pathophysiology observed here reflects the sever toxic effect from the crude extract of O. agardhii on both liver and kidney. Key words: Cyanobacterium, blood, histopathology, mice.
Highlights
Many species of bacteria secret varity of toxins (Osman et al, 2007; El-Menofy et al, 2014; Osman et al, 2015)
The haematological parameters investigated were red blood cell (RBC’s) count, hemoglobin content (HB), haematocrite value (HCT), mean cell hemoglobin concentration (MCHC), white blood cell count (WBC’s), platelets, serum glucose, albumin, cholesterol, triglycerides, urea, creatinine, lymphocyte; AST, and alanine aminotransferase (ALT) were measured to evaluate the pathophysiological changes induced by MCYST-LR
Histopathological results of mice liver exposed for prolonged duration of exposure of sub-lethal doses are presented in Figures 3 to 7
Summary
Many species of bacteria secret varity of toxins (Osman et al, 2007; El-Menofy et al, 2014; Osman et al, 2015). Cyanobacteria produces cyanotoxins source of natural product of toxins known as cyanotoxins, which might occur in fresh, brackish and marine water bodies. Poisoning cases are attributed to cyanobacterial toxins known since the late 19th century (Francis, 1878). Toxins-producing cyanobacteria pose a worldwide health threat to both human and animal due to their presence in both drinking and recreational water (Mutwally and Jamel Al-Layl, 1992; 1993; Ismail and Jamel Al-Layl, 1995; Jamel AlLayl, 1996; Dittmann and Wiegand, 2005; Boaru et al, 2006). Cyanobacterial produce microcystins toxic materials, which affect either the liver or the nervous system (Lawton and Codd, 1988; Mutwally and Jamel Al-Layl, 1992; 1993; Berry et al, 2008)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
