Abstract
Stemphylium solani isolates infect two varieties of eggplant, Solanum aethiopicum and Solanum melongena grown in Senegal (West Africa) and are morphologically quite similar. Using the random amplified polymorphic DNA (RAPD) procedure with arbitrary 10-mer primers, we were able to differentiate these S. solani isolates into two groups directly related to their plant host origin. A RAPD product of approximately 480 bases pairs obtained with OPF-20 primer were polymorphic between the two groups. Four new primers (F20F1, F20F2, F20R1 and F20R2) based on nucleotide sequence analysis of this 480 bases pairs RAPD fragment were developed. Such primers used pairwise amplified a single fragment from the DNA of S. solani isolates whatever their host origin. However, DNA extracted from Fusarium oxysporum (f. sp. vasinfectum, f. sp. elaeidis), Verticillium dahliae and Phyllosticta sp. isolates did not amplify using these primers. Our results indicate that these primers sets were good tools for specific identification of these two eggplants S. solani isolates by polymerase chain reaction (PCR). Key words: Stemphylium solani isolates, Solanum aethiopicum, Solanum melongena, random amplified polymorphic DNA (RAPD) markers, identification.
Highlights
Maymouna a Sy-Ndir1*, Komi Bru uno Assigb betse2, Mic chel Nicole e2, Tahir Ab bdoulaye D
Random amplified polymorphic DNA (RAPD) analyses used in this study appear to be extremely powerful and can separate individuals having intra specific variability
It gives more comprehensive information regarding the genetic variability among the fungal populations as it is based on the entire genome of an organism (Zimand et al, 1994; Achenbach et al, 1997; Mehta, 2001)
Summary
Maymouna a Sy-Ndir1*, Komi Bru uno Assigb betse, Mic chel Nicole e2, Tahir Ab bdoulaye D. Rec ceived 5 January, 2015; Accepted d 20 March, 2015 um solani is solates infec ct two varie eties of egg gplant, Solan num aethiop picum and Solanum. S (Wes st Africa) an nd are morp phologically quite simila ar. Using the e random amplified polymorphic p c DNA PD) procedu ure with arrbitrary 10-m mer primers s, we were able to differentiate e these S. so olani isolates into two groups g directtly related to o their plant host origin. A RAPD product of approximate ely 480 base es pairs obta ained with O. OPF-20 prime er were polym morphic bettween the two groups s.
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