Abstract
The conversion of glycerol to 1,3-propanediol (1,3-PD) is an environmentally friendly biological process and has attracted great interests worldwide. Efficient utilization of raw glycerol could bring significant economic benefits for 1,3-PD production. In the present study, the glycerol broth from strain Zygosacharomyces rouxii JL2011 was first used as the sole carbon source for 1,3-PD production by Klebsiella pneumoniae . The result indicates that the glycerol broth affected K. pneumoniae growth and 1,3-PD yield dramatically. Therefore, the raw glycerol was further prepared by extraction and purification, and the effect of raw glycerol on 1,3-PD production was investigated. The results obtained showed that the purified glycerol could be directly utilized in shake-flask culture by K. pneumoniae with a result comparable with that attained from pure glycerol. In addition, the fed-batch culture using raw glycerol as the substrate was conducted giving 56.1 g×L -1 with a yield of 0.40 g×g -1 glycerol and productivity of 1.12 g×L -1 ×h -1 . Key words: 1,3-propanediol, Klebsiella pneumoniae, Zygosacharomyces rouxii , raw glycerol.
Highlights
Glycerol is traditionally obtained as a by-product of fat hydrolysis in soap and other related materials production (Agarwal, 1990)
We investigated the feasibility of 1,3-PD production by Klebsiella pneumoniae using different glycerol preparation derived from Z. rouxii JL2011 as the substrate
To study whether glycerol broth from Z. rouxii JL2011 could be directly utilized in 1,3-PD production by K. pneumonia, the shake flask fermentation was performed
Summary
Glycerol is traditionally obtained as a by-product of fat hydrolysis in soap and other related materials production (Agarwal, 1990). The microbial conversion of glycerol to 1,3-PD is attractive because the process uses renewable feedstock and does not generate toxic byproducts (Biebl et al, 1999; Saxena et al, 2009). A cost reduction in 1,3-PD production can be achieved by using less expensive substrates It has been an economical and attractive route for 1,3-PD production using raw glycerol (Chatzifragkou et al, 2011; Gungormusler et al, 2011; Jun et al, 2010; Moon et al, 2010; Mu et al, 2006)
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