Abstract
The present research was planned tostudy the pharmacodynamic effects and mechanisms of terpenoids composition in Alisma orientale. In vivo test, the rat edema model was established with setting up the blank control group, the positive control furosemide group and high-dose, medium-dose and low-dose terpenoids groups. Collected rat urine after administration and detected the biochemical indexes of rat urine. Detected the expression of medullary aquaporin 2 (AQP-2) in the rat kidney by Western Blot and immunohistochemistry and detected the expression of AQP-2 mRNA by polymerase chain reaction (PCR). In vitrotest, using human tubular epithelial cell HK-2 model, the activity of HK-2 cells was determined with MTT colorimetric assay, the expression of AQP-2 protein in HK-2 cells was detected by Western Blot, the expression of AQP-2 mRNA was detected by RT-PCR, and IHC wasused to detect the relative expression of AQP-2. In vivotest showed that terpenoids composition in alisma can increase urine volume, promote electrolyte excretion in urine and decrease the expression of AQP-2 in rat kidney. Results in vitrotest showed that terpenoids composition cannot inhibit cells survival obviously but reduce the relative expression of AQP-2. Conclusively, terpenoids composition in alisma inhibited the expression of AQP-2, having diuretic effect.© 2021 Friends Science Publishers
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