Abstract
In this work, the effect of different concentrations of NaCl on calli induced from two sugarcane cultivars NCo310 and CP59-73 was studied. Growth and ion concentrations (Na+, Cl-, K+ and Ca2+) of calli were determined after 1, 2 and 3 months of stress with the objective to understand the cellular mechanisms operating in salt stress tolerance and to determine the implication of inorganic fraction in salt tolerance in sugarcane cultivars. A negative effect of the NaCl concentration and the duration of stress exposure on the callus rate growth was observed in both cultivars and with more extent in CP59-73 cv. Results showed an increase in Na+ and Cl– and a decrease in K+ and Ca2+ concentrations after 1, 2 and 3 months of salt stress exposure. It also showed that resistant cv. NCo310 stressed calli accumulated less Na+ and retained more K+ and Ca2+ than CP59-73 calli. Cl- appeared to be involved in osmotic adjustment since the resistant cv. NCo310 stressed calli accumulated more Cl- than CP59-73 ones. These results suggested that the resistance to salinity in sugarcane is associated with a high K+, Ca2+ and Cl- concentrations and a low Na+ concentration within cells. Key words: sugarcane (Saccharum sp.), salt stress, ion uptake, callus growth, long-term stress exposure.
Highlights
Salinity is a significant factor that affects crop production and agricultural sustainability worldwide, since about 10% of the land surface and 50% of all irrigated land in the world are prone to salinity (Flowers et al, 2010)
The calli obtained from both sugarcane cvs exhibited the same general tendency in response to NaCl concentration and to the duration of salt stress exposure
Similar results were obtain in rice (Lutts et al, 1996; Basu et al, 2002; Rattana and Bunnag, 2015), in borage (AlMohammed Maher et al, 2014), in alfalfa (Chaudhary et al, 1997), in safflower (Soheilikhah et al, 2015), in tomato (Rus et al, 2000), in potato (Forooghian and Esfarayeni, 2013) and in other sugarcane cultivars (Gandonou et al, 2005b)
Summary
Salinity is a significant factor that affects crop production and agricultural sustainability worldwide, since about 10% of the land surface and 50% of all irrigated land in the world are prone to salinity (Flowers et al, 2010).
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