Abstract
In the present study, the occurrence of Listeria monocytogenes, total coliforms and Escherichia coli in refrigerated raw milk from 75 dairy farms distributed in three regions (São Carlos- A, Pirassununga- B, and Piracicaba- C) of São Paulo State, Brazil was assessed. The production and storage conditions as well as milking procedures of raw milk were also evaluated. The analysis of L. monocytogenes was performed according to the method established by the Food and Drug Administration (FDA). The Official Method was used for the analysis of coliforms using the SimPlate system. L. monocytogenes was not isolated from raw milk samples (n=286), although Listeria innocua has been isolated in the milking environment. Total coliforms counts above 103 MPN/mL were found in 86% (n = 85), 75% (n = 71) and 72% (n = 66) of samples from regions A, B and C, respectively. E. coli was found in 66% of samples in region A, 65% in region B and 46% in region C. Of the 75 farms surveyed, 77.3% showed inadequate conditions for milk production as well as insanitary milking equipment and utensils which certainly compromises the quality of milk and dairy products. Key words: Listeria, Escherichia coli, milk quality, raw milk, microbiological quality.
Highlights
Escheriichia coli, c and prod duction n and s storag ge proc cesses s of o raw milk frrom da airy farrms in the sttate of
Monocytogenes, total coliforms and E. coli in samples of raw milk and their relation to different milking practices in small and intermediate scale dairy farms in São Paulo
Nine farms were selected for milk collection with a Moore’s strand (Lacen, 2000), which remained in the tank of raw milk for about 12 h before the procedure, performed in sterile packaging in order to increase the chance of isolation of L. monocytogenes
Summary
Escheriichia coli, c and prod duction n and s storag ge proc cesses s of o raw milk frrom da airy farrms in the sttate of. Received 10 March h, 2014; Accepte d 23 June, 2014 ent study, th he occurrenc ce of Listeria a monocytog genes, total c coliforms an nd Escherich hia coli in. In the prese refrigerated d raw milk frrom 75 dairy y farms distriibuted in thrree regions ((São Carlos-- A, Pirassun nunga- B, and Piracic caba- C) of São. L. mon nocytogenes s was not isolated fro om raw milk k samples (n n=286), altho ough Listeria ia innocua h has been iso olated in the e milking environmen nt. % showed inadequate conditions for milk pro oduction as well as insanitary milk king equipme ent and uten nsils which certainly compromis ses the qualitty of milk and dairy products.
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