Abstract
JiMaiTong (JMT), a Chinese herb compound prescription consisted of Flos chrysanthemi Indici, Spica prunellae and Semen cassiae for anti-hypertension. Luteolin is one of the major bioactivity compositions in F. chrysanthemi Indici in JMT. There are some reports about pharmacokinetics of luteolin in extract of F. chrysanthemi and husks of peanut in normal rats, but it lacked pharmacokinetic information of luteolin residing in a Chinese herb compound prescription in hypertensive animal models. The present study aimed to develop a high-performance liquid chromatography with photodiode array detection (HPLC-DAD) method for determination of luteolin in rat plasma and for pharmacokinetic study after oral administration of JMT to spontaneous hypertensive rats (SHR) and normal Sprague Dawley (SD) rats. After oral administration of JMT to SHR and SD rats, respectively the content of luteolin in blood samples at different time points were determined by a reversed-phase high-performance liquid chromatography (RP-HPLC) coupled with liquid-liquid phase extraction. This revealed a difference in disposition of luteolin in SHR and SD rats and a relationship between plasma concentration and hypertension of pathological state within the organism. This was the first report on the pharmacokinetic compare of luteolin between normal and hypertension pathological state rats. The hypertension pathological state would effects the disposition of luteolin, and it may provide a meaningful basis for the clinical application of JMT. Key words: Luteolin, pharmacokinetic, Flos chrysanthemi Indici, hypertension, Chinese herb.
Highlights
JiMaiTong (JMT) is a Chinese herb compound prescription which can effectively depress the systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial pressure (MAP) of spontaneous hypertensive rats (SHR) (Lv et al, 2010), map variability of renal hypertensive rats promote the microcirculation blood flow of RHR by improving nitrogen oxide (NO) content and depress ET-1 and Ang II level in serum (Chen et al., 2012)
JMT is composed of Flos chrysanthemi Indici, Spica prunellae and Semen cassia and contains flavonoids
Luteolin (3', 4', 5, 7-tetrahydroxyflavone) is one of the major bioactivity compositions in JMT; it acts as anti-hypertension by relaxing the vascular smooth muscle (Kim et al, 2006; Jiang et al, 2005; Duarte et al, 1993; Ichimura et al, 2006) and blood vessel (Jiang et al, 2005), promotes the opening of the cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel (Hou et al, 2008) and protects the cardiac muscle (Lv et al, 2010)
Summary
JiMaiTong (JMT) is a Chinese herb compound prescription which can effectively depress the systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial pressure (MAP) of spontaneous hypertensive rats (SHR) (Lv et al, 2010), map variability of renal hypertensive rats promote the microcirculation blood flow of RHR by improving nitrogen oxide (NO) content and depress ET-1 and Ang II level in serum (Chen et al., 2012). Chinese medicine would be different under the different physiological or pathological status (Zhao and Zhou, 2008) and the physiological disposition of the compositions in the drug would be changed as the same (Yang et al, 2005; Reng et al, 2006). These would induce the change of the plasma concentration and impact the pharmacological effects of the components. A comparative study of pharmacokinetics of compositions in drug in pathological pattern animals and normal animals has more practical significance for determining the efficacy material and guide the clinical application. The pharmacokinetic data could give us more information about the pharmacokinetics of JMT in vivo and give some reference for the clinical application
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