Abstract
Cleft palate (CP) is a common congenital defect. It causes serious problem to cleft-affected children. The gold standard of care is autogenous bone grafting which may cause additional problems together with long and extensive medical interventions. Tissue engineering is a promising solution for a widespread range of defects and disorders. It is reasonable to utilize this novel technology for CP management. Stem cells and growth factors play essential role in tissue engineering, so we evaluate effectiveness of adipose tissue and cord blood stem cells along with platelet rich plasma (PRP) on CP reconstruction. Human umbilical cord blood mononuclear cells (hUCB-MNCs) and human adipose-derived stem cells (hADSCs) were collected and incubated with Bromodeoxyuridine (BrdU) for labeling. The same was done to osteogenically differentiated hADSCs. Palatal bone defects were surgically made in rats. Afterwards, the labeled cells were mixed either with PRP or Aminoplasmal and injected to the defect borders. Immunohistochemistry and morphometry analysis were performed 4 weeks later. Data showed a significant difference in cleft size between cell-injected and control groups while the cleft site was filled with connective tissue rather than osseous tissue. Moreover, immunohistochemistry findings proved the presence of labeled cells in the surrounding tissue. These cells were detectable both in osseous and connective tissues. This study revealed the feasibility of stem cell and PRP application according to CP reconstruction. Hence, further investigation toward tissue engineering in CP may eliminate bone harvesting and its negative consequences. Key words: Cleft palate, platelet-rich-plasma, cell therapy, stem cells, tissue engineering.
Highlights
Cleft palate (CP) is a common congenital defect
Stem cells and growth factors play essential role in tissue engineering, so we evaluate effectiveness of adipose tissue and cord blood stem cells along with platelet rich plasma (PRP) on CP reconstruction
This study revealed the feasibility of stem cell and PRP application according to CP reconstruction
Summary
Cleft palate (CP) is a common congenital defect. It causes serious problem to cleft-affected children. During weeks 8 to 12 of human intrauterine life, palate is formed (palatogenesis) This structure separates oral and nasal cavities from each other. There was no immunological response to neither autologous nor allogeneic BM-MSCs and it showed both cells had the capacity to regenerate bone within craniofacial defects (Kok et al, 2003). Another study combined autologous BM-MSCs with sponge collagen protein and investigated restoration of the alveolar cleft in dog model by these materials. Results showed this method can be used clinically to treat alveolar cleft (Ou et al, 2007). Desirable results came from another study which used BM-MSCs seeded into different kinds of scaffolds for alveolar bone repairment in dog model (Mylonas et al, 2007)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
