Abstract
The present work attempts to investigate the ability of fusariumoxysporum and neurosporacrassa to ferment cellulose to ethanol directly. Batch experiments were performed for both the strain at 30.c in 250ml conical flasks.1000ml of production medium was distributed equally in 7 sterile,250 ml conical flasks, each containing various initial concentrations of cellulose 20,30,40,50,60,70 and 80g/l respectively.20ml of inoculum medium was transferred to each 100ml production medium in sterile conditions. Initial values of ethanol concentration, cellulose concentration and cell mass were determined. The inoculated flasks were maintained at 300 C in a rotary shaker operating at 200 rev min-1.Analyses were carried out for every 24 hours using the contents of the various conical flasks.Experiments were conducted for mixed cultures of different compositions 80:20, 60:40, 40:60, and 20:80by volume respectively.The cultures fusariumoxysporum and neurosporacrassa were mixed in proportion 80:20,60:40,40:60,and 20:80 respectively mixed cultures were used for inoculation.Based on the kinetic models, equations have been developed for the determination cell mass, product and substrate concentration as a function of time. Using the experimental data, constants of these equations have been evaluated. These mathematical relationships can be used with reasonable accuracy.
Published Version (Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: International Journal of Innovative Research in Science, Engineering and Technology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.