Abstract
Tissue culture technique can be used for normal callus culture and regeneration of a new clone. Nowadays the technique is used for establishment of dual culture of host and parasite, and regeneration of disease free plants. Normal callus was established and maintained on MS-medium supplemented with 6-benzylaminopurine (BAP, 1.0 mgl-1) and naphthalene acetic acid (NAA, 4.0 mgl-1). Efficient shoot bud and regeneration of cumin plant from callus was observed on combination of Kinetin (0.5 mgl-1) + indole-3-acetic acid (IAA, 1.0 mgl-1), BAP (0.1 mgl-1) + NAA (1.0 mgl-1) with 25 mgl-1 adenine sulphate (AS). Dual culture of Alternaria burnsii on cumin (Cuminum cyminum) callus was established on MS medium by using infected seeds. Dual culture was developed on MS-medium supplemented with NAA (4.0 mgl-1), BAP (1.0 mgl-1), biotin (1.0 mgl-1), thiamine hydrochloride (1.0 mgl-1), ascorbic acid (25.0 mgl-1) and casein hydrolysate (1.0 mgl-1). The dual culture developed from the infected seeds which were surface sterilized, indicates that the blight disease is basically seed borne in nature. Casein hydrolysate (1.0 mgl-1) and ascorbic acid (25 mgl-1) was found to be best for the growth of the fungus in the dual culture. It was concluded that the study can be used for disease free plants and enhance the growth of the fungus through control the nutrients from the host during disease development. Key words: Cumin, Alternaria burnsii, callus, dual culture.
Highlights
Cumin num cy yminum m L.) p plants from callus c and a es stablishmentt of dual cultture off host an nd parrasite
For normal callus cultures and regeneration, the certified seeds of Cuminum cyminum variety RZ-19 were surface sterilized with 0.1%
For the auxins viz., indole-3-acetic acid (IAA), naphthalene acetic acid (NAA), indole butyric acid (IBA) and 2,4-dichlorophenoxy acetic acid (2,4-D), stocks were prepared by dissolving them in a few drops of absolute alcohol initially
Summary
Cumin num cy yminum m L.) p plants from callus c and a es stablishmentt of dual cultture off host an nd parrasite In vitro culture of plant tissues provides an excellent system for the study of growth, metabolism and morphogenesis. Dual cultures have been used frequently in plant pathological studies for growth of biotrophic fungi and in vitro expression of disease resistance (Goyal, 1990; Chorabik, 2014). Tissue culture techniques are being used widely as tool in crop improvement by generation of novel disease resistant lines of crop plants (Garg et al, 1986). (2003) studied the effect of cultural filtrate of A. brassicae on biochemical constituents of calli of Brassicas In this experiment the tissue culture technique was used to study the isolation and establishment of normal callus culture, regeneration of cumin plant from callus and dual culture of host and parasite
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