Abstract
The marine biofilm bacteria were isolated on polyvinyl chloride (PVC) sheet immersed from offshore platform of Central Electrochemical Research Center (CECRI), Tuticorin, Tami Nadu. The isolated marine biofilm bacteria such as Pseudomonas sp., Aeromonas sp., Enterobacter sp., Bacillus spp., Flavobacterium sp., Microccocus sp. and Cytophaga sp were identified up to generic level. The Pseudomonas sp. was the dominant, primary biofilm forming bacteria. The cell free crude extract of single (Aeromonas sp.) and co-cultured (Aeromonas sp. + Enterobacter sp.) system showed the significant repellent activity against Pseudomonas sp. The crude fractions were characterized by FTIR for functional group identification and GC-MS for compound identification. The results of FTIR shows different peaks like 3302.16, 2816.56, 1227.44 and 2724.02 cm–1 indicating the presence of bromine, amine, and phenolic, hydroxyl, amino, carbonyl and phosphoryl functional groups. It was also noted that mixed culture system produced increased level of bacteriocin as compared to single culture system. The chemical characterizations of the inhibitory molecules such as nonane, is common to both single as well as co-culture system. The higher inhibitory activity observed in co-culture system is due to the presence of nonadecane, heneicosane and cholestane compounds. The natural biogenic compounds from the marine bacteria studied have the potential to be used as a substitute to commercial biocides for anti-microfouling. Key words: Anti-biofilm activity, bacteriocin, nonane, bioactivity, bioremediation.
Highlights
Chemic cal cha aracterrization n and bioacttivity e evaluattion of bactteriocin from m marin ne bioffilm-fo orming g bacte eria
Enterobacter s sp., Bacillus spp., Flavob bacterium sp., Microccocus sp. an nd Cytophag ga sp were identified up p to generic llevel
For ba acteriocin produ uction, the isola ates were inocu ulated by means of (i) single cell cu ulture by Aerom monas sp. and d co-culture by
Summary
INT ps, marine pla atforms, offsho ore Marine structures such as ship rigss and jetties are a under con nstant attack from f the marine envvironment by fouling organ nisms These structures ne eed to b be protected from f the influe ences of the key elements s of the marine enviironment suc ch as saltwatter, temperatu ure uations and b biofouling (Ch hambers et all., 2006). Bio ofluctu foulin ng is one of tthe major sig gnificant problems and ubiiquitou us in the marrine environm ment It is a na atural process of collonization of ssubmerged su urfaces, eithe er living or artiificial, involving a w wide range of m micro and macro organisms s. The main p purpose of the e prese ent study wass to screen tthe antifoulin ng potential of o marin ne bacterial metabolites aga ainst primary b biofilm forming g bacte eria.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
