Abstract
Indigenous strains of Trichoderma viride (ITCC No. 6889), Pseudomonas fluorescens (ITCC No. B0034) and Purpureocillium lilacinum (ITCC No.6887) were isolated from undisturbed forest eco-system of Southern India. These three bio-mediators were evaluated for their antagonism towards root knot nematode, Meloidogyne incognita and Fusarium oxysporum f.sp. conglutinans in vitro. Cell free culture filtrate of these strains significantly inhibited the egg hatching and caused juvenile (J2) mortality of M. incognita at 25, 50, 75 and 100% concentrations. Maximum inhibition in egg hatching and juvenile mortality were recorded in P. lilacinum as 94.21 and 91.28%, respectively after 120 h. It was followed by T. viride and P. fluorescen s which recorded 92.72 and 91.46% and 89.12 and 90.14% inhibition in egg hatching and juvenile mortality, respectively after 120 h. Antagonism of T. viride on F. oxysporum was recorded maximum on the 5th day as 45.82%. Similarly, the antagonism on the 5th day for both the bioagents of P. lilacinum and P. fluorescens were recorded as 45.26 and 44.19%, respectively. Key words : Biocontrol agents, culture filtrate, Fusarium oxysporum , Meloidogyne incognita .
Highlights
Root-Knot nematodes are causing a notable damage to a wide range of vegetable crops causing significant yield loss in tropical and sub-tropical agriculture (Sikora and Fernandez, 2005)
Indigenous strains of Trichoderma viride (ITCC No 6889), Pseudomonas fluorescens (ITCC No B0034) and Purpureocillium lilacinum (ITCC No.6887) were isolated from undisturbed forest eco-system of Southern India. These three bio-mediators were evaluated for their antagonism towards root knot nematode, Meloidogyne incognita and Fusarium oxysporum f.sp. conglutinans in vitro
T. viride and P. lilacinum matching to National Centre for Biotechnology Information (NCBI) were 94 and 96%, respectively and P. fluorescens was 97%
Summary
Root-Knot nematodes are causing a notable damage to a wide range of vegetable crops causing significant yield loss in tropical and sub-tropical agriculture (Sikora and Fernandez, 2005). The symptoms of nematode disease are manifested by the formation of root galls accompanied by stunted growth, chlorosis and loss of viability of the plant (Babu et al, 1999). F. oxysporum has a worldwide distribution and causes severe root rot or vascular wilt in ample range of plant families (Enya et al, 2008; Lievens et al, 2008; Michielse and Rep, 2009). This fungal pathogen infects the seed and early stages of seedling growth, causing seed decay and damping-off (Punja et al, 2004).
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