English

Abstract

The natural pyrethroids are not so much effective against agricultural pests, thus artificial synthesis of synthetic pyrethroids like fenvalerate was carried out by scientists. Fenvalerate is a insecticide has been proved to be highly toxic to non-target species like amphibians. In our study, Healthy frogs, Rana tigrina weighing 50 ± 3 gms were collected from the pond, acclimated to the laboratory conditions in large glass aquaria with water. The frogs were divided into groups of ten animals. They were exposed to different pesticide concentrations of fenvalerate, both commercial and technical grade, according to biomass ratio as suggested by Doundroff et al., (1951). The Total carbohydrate content in the present study Control Muscle tissue has shown higher amount of carbohydrates than compare with Brain and Intestine tissue. Under fenvalerate intoxication, the frog tissues showed consistent decrement in all the periods and it may be due to rapid utilization to withstand pesticide of stress. Total protein contents in control frog tissues the Muscle showed higher concentration of proteins than Intestine and Brain. The protein content in experimental frog tissues showed depleted levels when compared to controls. The significant decrease in protein and it may be due rapid utilization to withstand pesticide of stress. Total free amino acid (FAA) showed enhanced Intestine FAA levels, the levels in fenvalerate exposed frog tissues were increased significantly when compared to control. These elevated levels may be due to improved protease activity or enhanced transamination. From the above observations fenvalerate seems to be hazards to the aquatic life, causing severe biochemical changes and permanent architectural changes investigated in the present investigation.