Abstract
Trehalose is a non-reducing disaccharide which consists of two glucose units that functions as a compatible solute to stabilize the membrane structures under heat and desiccation stress. Trehalose-6-phosphate synthase (TPS) and trehalose-6- phosphate phosphatase (TPP) are the key enzymes for trehalose biosynthesize in the plant kingdom. On the basis of bioinformatics prediction, fragment containing an open reading frame of 945 bp was cloned from durum wheat. Sequence comparison and analysis of conserved domains revealed the presence of a TPP domain. Full length of the gene was isolated using gene race technology. Semi-quantitative RT-PCR and real time quantitative PCR indicated that the expression of this gene is up-regulated in response to drought stress. The biochemical assay of the trehalase activity showed that the enzyme's activity decreased under the dehydration stress. The obtained phylogenic tree showed that the isolated TPP protein forms a distinct clad close to the Oryza sativa trehalose-6- phosphate phosphatase. In silico and comparative mapping indicated that the isolated TPP gene is localized on rice chromosome 8, durum wheat chromosome 20, bread wheat chromosome 3B, oat linkage group E, sorghum chromosome 4 and barley 5H. Key words: Abiotic stress tolerance, trehalose-6- phosphate phosphatase (TPP), durum wheat, trehalose, real time PCR, cloning, full length gene, drought stress.
Highlights
The global food situation is currently being redefined by many driving forces like globalization, urbanization, energy prices, and climate change
One of the required actions that is suggested by the Egyptian Cabinet, Information and Decision Support Center (IDSC) to solve the food problem in Egypt was to focus on the agricultural research to enhance the capability of crop plants to withstand different abiotic stresses, such as salt, drought, 10 Int
This work aimed at the isolation, cloning and characterization of functional trehalose-6-phospate phosphatase (TPP) gene from durum wheat under dehydration stress to investigate the trehalose 6 phosphatase (TPP) gene ability for drought tolerant in Durum wheat in order to examine the magnitude of the trehalose-6- phosphate phosphatase (TPP) gene response to drought stress
Summary
The global food situation is currently being redefined by many driving forces like globalization, urbanization, energy prices, and climate change. According to the report of the food and agriculture organization of the United Nations (FAO) 2010, the number of undernourished people around the world in 2010 has declined but remains abnormal and unacceptable. The renewed global attention is being given to the role of agriculture and food in development policy. One of the required actions that is suggested by the Egyptian Cabinet, Information and Decision Support Center (IDSC) to solve the food problem in Egypt was to focus on the agricultural research to enhance the capability of crop plants to withstand different abiotic stresses, such as salt, drought, 10 Int. J.
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