Abstract

The exploration and use of lipases produced by microorganisms are in line with societal concerns since they provide clean development options within the standards of environmental sustainability. The objective of this work was therefore to isolate microorganisms with the potential of producing lipases, which could be used in the waste water treatment systems of slaughterhouse units. Waste water samples were collected from the treatment lagoons of a poultry slaughterhouse. The PCA culture medium was used for the isolation of fungi. The pH was adjusted to 6.5 and autoclaved at 1 atm of pressure, for a time of 30 min. The isolated and purified colonies were evaluated in order to obtain lipase producing strains. The results obtained in this study enabled the conclusion that the waste water produced in slaughterhouse has a high microbial load, highlighting bacteria with 6.8 × 108, filamentous fungi with 3.4 × 104 and yeasts with 2.2 x 102 CFU.ml-1. Out of this microbial population, six species of lipase producing fungi were isolated, with the fungi Aspergillus flavus and Aspergillus terreus being those that obtained the highest enzymatic indices. Key words: Lipase, fungi, effluent, lipids, enzymes.

Highlights

  • Industrial processes are one of the major factors responsible for water pollution and contamination through the untreated release of effluents into natural waterways, causing significant damage to the environment and the population

  • The objective of this work was to isolate microorganisms with the potential of producing lipases, which could be used in the waste water treatment systems of slaughterhouse units

  • The results obtained in this study enabled the conclusion that the waste water produced in slaughterhouse has a high microbial load, highlighting bacteria with 6.8 × 108, filamentous fungi with 3.4 × 104 and yeasts with 2.2 x 102 CFU.ml-1

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Summary

Introduction

Industrial processes are one of the major factors responsible for water pollution and contamination through the untreated release of effluents into natural waterways, causing significant damage to the environment and the population. Considering the large number of companies that still dispose of their effluent without any kind of treatment into the waterways, the contribution of these industries to water pollution is quite significant. This waste is highly putrescible and starts to decompose in a few hours, forming gases that produce bad odors that make it difficult to breathe in the establishment's surroundings, causing nuisance to the local population. These effluents are responsible for the bad image that the public has of these establishments

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