Abstract
shown to inhibit the activity of fungal endopolygalacturonase (endo-PGs) and modulate their activity and has the potential to be developed as a disease or resistance biomarker for the oil palm. The identification and isolation of this gene in oil palm allowed for the study of its differential expression during the fungal infection. The oil palm PGIP gene (EgPGIP) has between 60-100% similarities with the database sequence of PGIP from other monocotyledons. Interestingly, we found that the expression of EgPGIP gene measured using Real-Time PCR showed that the expression level of EgPGIP in infected oil palm was temporally down regulated. The results suggest that, down regulation of the EgPGIP is related to the establishment of infection by G. boninense.
Highlights
Extensive studies to identify changes in gene expression during pathogen-host interaction have enhanced discovery of genes regulating the plant defence response to pathogen attack and can lead to the understanding of why other plants are susceptible
The results suggest that, down regulation of the EgPGIP is related to the establishment of infection by G. boninense
The putative oil palm EgPGIP gene was successfully amplified from the genomic DNA (1.97 mg/ml and purity 1.83 A260/A280) by PCR
Summary
Extensive studies to identify changes in gene expression during pathogen-host interaction have enhanced discovery of genes regulating the plant defence response to pathogen attack and can lead to the understanding of why other plants are susceptible. There is an indication that there are differences in susceptibility to BSR between germplasm materials from different genetic origins (De Franqueville et al, 2001). There is interest in developing diagnostic tools such as using PCR primers for detection of the pathogen in oil palms (Bridge et al, 2001). Development of molecular biology studies of oil palm towards the understanding of the defence and stress response mechanism may be a. Lack of understanding and molecular information about disease establishment, development and spread has hampered the study of the molecular interaction between the fungus and its host (oil palm). Quantitative methods for global and simultaneous analysis of expression profiles, such as Real-Time PCR would be powerful in giving overall understanding of the molecular and biochemical basis of the response to infection by Ganoderma (Ravigadevi et al, 2005)
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