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Abstract
Local soybean cultivar “Nannong 94-16” was treated with NaN 3- 60 Co ray and EMS separately, and two curled cotyledon mutants were obtained. Local soybean cultivar “TSB” was treated with 60 Co ray and one curled cotyledon mutant was obtained. Two-dimension electrophoresis analysis was employed to determine the differential proteins between three mutants and their wild types. The results showed that there were three common differential protein spots between the mutants and their wild types. Further analysis suggests that the two increased protein spots in mutants were both globulin subunit G3/A1aB1b, while the decreased protein spot in mutants was lectin. These results indicate that there may be an association between protein components (glycinin subunits G3/A1aB1b, lectin) and the curled cotyledon phenotype in soybean.
Highlights
Soybean [Glycine max (L.) Merri.] is one of the most economically important crops in the world (Wilcox, 2004)
The results indicated that there were two increased protein spots of all mutants compared to their wild types; these spots were marked by squares; and there was one decreased protein spots of all mutants comparing to their wild types, marked by a circle (Figure 2)
The three protein spots that were discovered from 2-D gels may be correlated with curled cotyledon mutants
Summary
Soybean [Glycine max (L.) Merri.] is one of the most economically important crops in the world (Wilcox, 2004). Accumulation of specific proteins and other compounds for nutrient storage to high levels is one of the characteristic events during seed development. Improvement of storage protein in seed is being given more and more attention all over the world (Kim et al, 1990). Cotyledon is one of the most important organs of growth and metabolism for seeds germinating. Many important discoveries have been obtained from mutant research. Some research about the characters of cotyledon mutant in many plants is developing. There have been a few reports related to cotyledon mutant in soybean. The curled cotyledon soybeans used in this research are novel soybean discovered through physical and chemical mutagenesis. For a better understanding of the consequences of genetic phenomena, elucidation of the protein composition is necessary because of its direct relationship to phenotype (Skylas et al, 2000)
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