Abstract
Currently, natural products have been evaluated as sources of antimicrobial agents. Considering the increasing use of pomegranate, it has become important to establish a correlation between the phytocompounds and the antimicrobial properties of the crude extract used in Brazilian folklore medicine. The compositional analysis revealed the presence of classes of secondary metabolites of pharmaceutical interest, among them, tannins, flavonoids and phenolic compounds. The in vitro study was performed by broth micro-dilution susceptibility assay according to the protocols of the National Committee for Clinical Laboratory Standards and described the antibacterial and antifungal activities of the extract. The preeminent antimicrobial activities were recorded against Staphylococcus aureus and Staphylococcus epidermidis. In order to estimate the ex vivo antimicrobial activity of the extract, serum and granulomatous tissues were separately submitted to microbiological assay. The crude extract did not present any inhibition zone. Thus, it is possible to suggest that after the oral ingestion of the extract, bacteria present in the gastrointestinal tract of the animal, hydrolyze phytocompounds, which present antimicrobial potential in in vitro models but do not present sufficient serum and tissue concentrations to exert this activity in ex vivo and presumably in in vivo models. Key words: Punicaceae, Punica granatum L., preclinical, antimicrobial, in vitro, ex vivo.
Highlights
Punica granatum L. (Punicaceae) is a large deciduous shrub or small tree which fruit, are common in the Mediterranean and has been therapeutically used as food in Brazil
The crude extract was preliminarily subjected to a qualitative analysis to detection of the secondary metabolites classes by chemical reactions characteristic for each substances (Delgado et Twenty-four adult male Wistar rats (Rattus norvegicus albinus), weighing 175 ± 25 g, were acquired from the biotery of ABC
A compositional analysis has been accomplished to verify the presence of major classes of secondary metabolites of pharmaceutical interest in the crude extract from the fruits of P. granatum L. and these phytochemical results were attached to its preclinical antimicrobial evaluation
Summary
Punica granatum L. (Punicaceae) is a large deciduous shrub or small tree which fruit (pomegranate), are common in the Mediterranean and has been therapeutically used as food in Brazil. (Punicaceae) is a large deciduous shrub or small tree which fruit (pomegranate), are common in the Mediterranean and has been therapeutically used as food in Brazil. The fruit is delimited by a leathery pericarp contained within are numerous arils, each as a single seed surrounded by a translucent juice-containing sac. Thin acrid-tasting membranes extend into the interior of the fruit from the pericarp, providing a lattice work for suspending the arils.
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