Abstract
FT is thought to be the florigen in plants. In this research, a new method for promoting lily flowering was introduced. The function of FT gene cloned fromArabidopsis on promoting lily flowering was analyzed. pET-30a-FT vector was constructed to indicate the expression of FT:eGFP fuse protein in prokaryotic cells. FT:eGFP was also constructed into plant virus vector-pGR106. AgrobacteriumGV3101 harboring pGR106-FT was injected into lily. The injected lily showed early flowering when compared with the control plants. The detection of eGFP and PCR analysis indicated that the virus harboring FT:eGFP was replicated and expressed in the host plants. The results showed that FT:eGFP fuse protein functioned in promoting lily flowering. Key words: FT, viral vector, lily.
Highlights
The transition from vegetative phase to reproductive growth is controlled by day length in many plant species
Florigen is produced in the leaves under inductive day length conditions and transported to the shoot apex where it interacts with the other proteins and reprograms the SAM to form flowers
In order to prove the fluorescent character, the FT:eGFP fuse protein, the recombinant E. coli and the purified fuse protein were detected under fluorescent microscope (Figure 1)
Summary
The transition from vegetative phase to reproductive growth is controlled by day length in many plant species. Studies on Arabidopsis and rice showed that FT protein and the rice Hd3a participated in long-distance signaling to induce flowering. The function of FT:eGFP fuse protein was studied in lily via virus vector injection, and its corresponding results were analyzed. In order to detect FT:eGFP DNA fragment, genomic DNA was extracted from the injected lily leaves and control leaves, and primers were synthesized as follows: forward primer from FT gene, 5’ TTCCCGTGGCCTCAGTTT3’; reverse primer from eGFP gene, 5’ AGTTCACCTTGATGCCGTTC-3’.
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