Abstract
The existence of a target DNA fragments associated with the potential high level ofcatechins in gambier plant was carried out preliminary using RAPD markers. Targetfragment was ligated and transformed into Escherichia coli DH5α strains usingpGem-T Easy vector. The purpose of this study was to obtain information of specificDNA fragments and to generate specific primer combinations associated with potentially high content of catechins. DNA sequence was successfully generated with206 nucleotides in length. The combination of specific primers, designated as Udtg3(5'-CAGATCCTTTGCCACACTGA-3') and Udtg4 (5'- CGTACCTGCCAA CATA ACAG- 3') were successfully designed and could be applied as a diagnostic marker indetection of catechin content production of gambier plant. Key words: Gambier, RAPD, specific primers, MAS, catechin.
Highlights
Gambier plants (Uncaria gambier (Hunter) Roxb.) is one of the export of plantation commodities possesing high economic value and this is based on their commercial role
In order to speed up selection step, random amplified polymorphic DNA (RAPD)-based
Gambier extract contains several important components which are useful for health and pharmaceutical process
Summary
Gambier plants (Uncaria gambier (Hunter) Roxb.) is one of the export of plantation commodities possesing high economic value and this is based on their commercial role. The gambier resin contains mainly catechins that is necessary in the pharmaceutical industries, cosmetics, paint, and others. Fauza (2009) described four genotypes of gambier mainly found in West Sumatera, namely: Udang, Cubadak, Riau Gadang dan Riau Mancik. Among this genotypes, Udang is the most productive genotype than the other three based on their sap and catechin content yield (Hasan et al, 2000). Udang is the most productive genotype than the other three based on their sap and catechin content yield (Hasan et al, 2000) This was further proved by Ferita et al (2009) that they showed range productivity of
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