Abstract
Murashige and Skoog (MS), Knudson C (KC) and Vacin and Went (VW) nutrient media are among the most widely used for micropropagation of orchid species. Therefore, this study was conducted to evaluate the effect of these growth media on in vitro shoot and root proliferation of Vanilla planifolia by using nodal segment as explants. Three different media; Murashige and Skoog (MS), Knudson C (KC) and Vacin and Went (VW) were supplemented with 0.5 mg/L of α-naphthalene acetic acid (NAA) and 1.0 mg/L of 6-benzylaminopurine (BAP) respectively to enhance the plant growth. The findings showed that the explants developed in MS medium yield the highest increase in length of shoot and root, with the average of 2.37 ± 0.76 cm and 2.04 ± 0.95 cm respectively. Apart from that, the plantlets in MS medium have well-formed leaves and extended roots. On the other hand, plantlets in both KC and VW media showed lower average of shoot and root length as compared to those cultured on MS medium. The length of shoot and root initiated from the nodal segments of V. planifolia showed significant difference when cultured in different types of nutrient media. Key words: Vanilla planifolia, micropropagation, regeneration, in vitro; tissue culture.
Highlights
Tropical orchid, Vanilla planifolia belongs to the family Orchidaceae and is native to southeastern Mexico (Purseglove et al, 1981)
The findings showed that the explants developed in Murashige and Skoog (MS) medium yield the highest increase in length of shoot and root, with the average of 2.37 ± 0.76 cm and 2.04 ± 0.95 cm respectively
The effect of plant growth media on shoots and root proliferation of Vanilla planifolia The study showed that MS medium was the most effective for the development of shoots in V. planifolia, followed by Knudson C (KC) (Knudson, 1946) and VW (Vacin and Went, 1949) media
Summary
Vanilla planifolia belongs to the family Orchidaceae and is native to southeastern Mexico (Purseglove et al, 1981). The main producers of the vanilla plants are Madagascar, Indonesia, Mexico, Tahiti and India (Shruthi and Naira, 2013). The main product from this orchid plays a major positive role in the economy of several countries. V. planifolia are commercially cultivated for vanillin which is found in the beans. This substance is widely used as flavoring in foods, drinks and fragrance industries. Vanilla is involved in the production of pharmaceutical and cosmetics (Hilda et al, 2008).
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