Abstract
Proper conditioning of leaf tissues in collection expeditions can affect the quantity and quality of DNA in the extraction process. The aim of this work was to define a method of preserving foliar tissue suitable for obtaining DNA from Brazilian Cerrado trees. Young leaves of species (Mangaba and Baru) were collected and conditioned in five different treatments during a period of six days. Genomic DNA was obtained using two alternative versions of the cetyltrimethyl ammonium bromide (CTAB) protocol. For Mangaba, no statistical differences were verified between means of DNA values ​​obtained with diversity arrays technology (DArT) (55 ng/μL) and CTAB (48 ng/μL) methods. It was found that the amounts of DNA obtained with the methods used differed according with the conditioning type and time (F20,60 = 1.98; p = 0.022). For Baru, the mean of DNA extracted was significantly higher (F1,60 = 42.81; p < 0.01) from the CTAB method (80 ng/μL). A significant difference (p < 0.05) was also observed between DNA means of conditioning types (F4,60 = 1.1, p = 2 × 10-4), without this being detected over time. Any preservation method tested is indicated for the selection of Mangaba and Baru foliar tissue conditioning for DNA extraction in a short period (up to six days). Key words: Conservation, germplasm, native tree, DNA purification.
Highlights
The Cerrado is considered one of the world's biodiversity hotspots, housing 1/3 of the Brazilian biota and 5% of the world's flora and fauna (Eiten, 1994; Sloan et al, 2014)
Five treatments were defined based on the methods of preservation of the samples of leaves used for collection expeditions until the moment of extraction, such as: 1) TE buffer; 2) TE buffer with ice and maintained at -20°C (TEBI); 3) with ice alone and maintained at -20°C (I); 4) only with silica gel; 5) without substance (WS)
Comparing the Mangaba foliar tissues submitted to the five methods of preservation, through the time intervals between the extracted DNA, visual changes were verified in the conditioned samples
Summary
The Cerrado is considered one of the world's biodiversity hotspots, housing 1/3 of the Brazilian biota and 5% of the world's flora and fauna (Eiten, 1994; Sloan et al, 2014). In the area, it is the second largest vegetation cover in Brazil and South America (Mendonça et al, 1998; Sano et al, 2010) with a high impact on food security (FAO, 2015).
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
