Abstract
Antibacterial properties of Lactobacillus strains isolated from traditional fermented mustard were investigated. Among the strains tested, strain B0105 was found to produce bacteriocin-like compounds against Streptococcus mutans BCRC 10793. Strain B0105 was identified as Lactobacillus plantarum by phenotypical and physiological tests as well as16S rDNA identification. The molecular weight of bacteriocin-like compounds was 3.5 and 4.7 kDa by Tris-Tricine SDS-PAGE. In addition, this bacteriocin-like exhibited a strong antibacterial activity, heat stability (15 min at 121°C) and pH stability (pH 2.0–4.0) againstS. mutans BCRC 10793, however, it was sensitive to proteolytic enzyme. Overall, the results obtained, demonstrate that strain B0105 is able to produce bacteriocin-like compounds inhibiting S. mutans BCRC 10793, making it potential candidate for antibacterial agents. Key words: Lactobacillus plantarum, bacteriocin, Streptococcus mutans.
Highlights
Dental caries is influenced by diverse bacterial, dietary, environmental, socioeconomic and physiological risk factors and is one of the most prevalent diseases in humans (Sánchez-Pérez et al, 2004; Marthaler, 2004)
The antibacterial activity of B0105 and B0158 isolates against S. mutans was maintained after adjustment of pH and removal of catalase, indicating that the antibacterial agents of B0105 and B0158 could be bacteriocin-like compounds
From isolated lactic acid bacteria (LAB) strains, strain B0105 which produced a bacteriocin-like compound which strongly inhibited S. mutans was isolated from traditional fermented mustard
Summary
Dental caries is influenced by diverse bacterial, dietary, environmental, socioeconomic and physiological risk factors and is one of the most prevalent diseases in humans (Sánchez-Pérez et al, 2004; Marthaler, 2004). One of the properties required for a caries-preventive sweetener is that it decreases fermentation of sugar to acid by oralmicroorganisms, including Streptococcus mutans which is a primary causative agent of human dental caries (Padilla et al, 2006). To control dental caries formation, antibacterial agents that reduce the number of S. mutans are commonly used, for exam-ple xylitol, chlorhexidine and triclosan (Fejerskov, 2004). Using such antibacterials may induce resistance in micro-organisms (Badet et al, 2004; Diekema et al, 2004). It is important to search for new antibacterials that may control the effect of S. mutans (Padilla et al, 2006)
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