Abstract
Saccharomyces cerevisiae has always gained a huge amount of attention due to its extensive and enormous application value. Various methods are used to optimize the fermentation characteristics of S. cerevisiae. In this work, we investigated the fermentation capacity and stress responses on a physiological level of S. cerevisiae which affected by moderate pulsed electric field (PEF, 1 to 3 kV·cm-1). The fermentation capacity was illustrated by glucose consumption and metabolites yield (ethanol and glycerol). The expressions of ten glycolysis-associated genes were studied to elucidate the fermentative processes. The results showed that fermentation capacity of S. cerevisiae was promoted by moderate PEF treatments, with higher glucose consumption and ethanol yield. Yeast cells also gained a faster growth rate under constant PEF stimulation. Analysis of gene expression involved in glycolytic pathway showed that moderate PEF treatments induced a higher glycolytic flux, especially in terms of synthesis of ethanol. It would be a promising technique for PEF to enhance fermentation capacity and growth of S. cerevisiae. © 2021 Friends Science Publishers
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
