Abstract
The objective of this study was to establish an efficient and reproducible in vitroplant regeneration for Citrullus lanatus cv. Zaojia. To achieve optimal conditions for adventitious shoot induction, five explants (entire cotyledons, distal cotyledons, proximal cotyledons, cotyledonary node A and cotyledonary node B) were tested on MS medium supplemented with different concentrations and combinations of growth regulators (0 to 0.2 mg/L IAA and 1.0 to 5.0 mg/L BA), the results showed that entire cotyledons cultured in MS + BA (2.0mg/L) + IAA(0.2mg/L) achieved the highest regenerated rate (89.67%) and the optimal protocol screened in this experiment had 7.69 ± 0.10 shoots per explants. Adventitious shoots were able to elongate both on MS medium with 0.2 mg/L KT and 0.2 mg/L NAA; IBA 0.3mg/L was found to be effective in the production of root. Acclimatized plantlets transferredto pot resumed growth, and their stems and leaves elongated and expanded in one month. Key words: Watermelon (Citrullus lanatus Thumb.), optimized system, regeneration, cotyledon explants, cotyledonary node.
Highlights
Watermelon (Citrullus lanatus Thumb.) is an important cucurbit crop species and it is a popular fruit which is native to Central Africa
To achieve optimal conditions for adventitious shoot induction, five explants were tested on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of growth regulators (0 to 0.2 mg/L indoleacetic acid (IAA) and 1.0 to 5.0 mg/L BA), the results showed that entire cotyledons cultured in MS + BA (2.0mg/L) + IAA(0.2mg/L) achieved the highest regenerated rate (89.67%) and the optimal protocol screened in this experiment had 7.69 ± 0.10 shoots per explants
Explant type has shown to be important for morphogenesis, since competent cells for adventitious shoots formation in cucurbits seem to be restricted to specific cotyledon regions (Choi et al, 1994; Compton, 2000; Ananthakrishman et al, 2003)
Summary
Watermelon (Citrullus lanatus Thumb.) is an important cucurbit crop species and it is a popular fruit which is native to Central Africa. It was first grown by ancient Egyptians and is believed to have been cultured in Asia minor, Russia and the near and Middle East, thousands of years ago. The soluble fiber in watermelon may help to reduce cholesterol and risk of heart disease. It is a good source of fiber, which is important for keeping digestive track operating properly by preventing constipation, hemorrhoids and diverticular diseases. The nutritive value of the seeds is due to their high oil and protein content
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