Abstract

The objective of this work was to evaluate the use of ten types of grasses as base substrate for the cultivation of strain GLM-10/02 of Ganoderma lucidum, considering the chemical characterization of the substrates, the biological efficiency (BE), the quantification of fresh and dry basidiomata and the number of basidiomata as evaluation criteria. Ten treatments were outlined, made up with napier or elephant grass (Pennisetum purpureum), marandu grass (Brachiaria brizantha cv. Marandu), aruana grass (Panicum maximum cv. Aruana), massai grass (Panicum maximum cv. Massai), mombaca grass (Panicum maximum cv. Mombaca), brachiaria grass (Brachiaria decumbens cv. Basilisk), humidicola (Brachiaria humidicola cv. humidicola), xaraes grass (B. brizantha cv. Xaraes), tifton (Cynodon species cv. Tifton 85), piata grass (B. brizantha cv. BRS Piata) and a control treatment based on eucalyptus sawdust. All the treatments had 80% of grass, 18% of wheat bran and 2% of limestone, with humidity adjusted to 60%. The treatments based on B. brizantha cv. Aruana (aruana) and Cynodon spp. cv. Tifton 85 (tifton) showed the best results, with 22.9 and 25% of BE, respectively. These data showed that not all grasses used in the experiment have the same fungal biomass conversion and it was concluded that B. brizantha cv. Aruana (aruana) and Cynodon spp. cv. tifton 85 (tifton) were the most indicated grasses for the cultivation of G. lucidum. Key words: Productivity, biological efficiency, substrates, fungi.

Highlights

  • The appropriate selection of the substrate is very important for the success of every kind of mushroom production

  • The objective of this work was to evaluate the use of ten types of grasses as base substrate for the cultivation of strain GLM-10/02 of Ganoderma lucidum, considering the chemical characterization of the substrates, the biological efficiency (BE), the quantification of fresh and dry basidiomata and the number of basidiomata as evaluation criteria

  • The spawn of strain GLM-10/02 of G. lucidum was obtained from the Fungi Herbarium of the Mushrooms Module, FCA/Universidade Estadual Paulista (UNESP) and was wrapped in High Density Polyethylene (HDPE) packs containing the substrates previously prepared (Table 1) and sterilized in an autoclave at a temperature of 121°C for 4 h

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Summary

Introduction

The appropriate selection of the substrate is very important for the success of every kind of mushroom production. Agro-industrial residues, such as grasses, coffee pulp, cereals bran, crushed sugar cane, processed fruit peels, potato, cereals flour, cassava and others, are widely used substrates in these processes (Alquati et al, 2016; Carvalho et al, 2015; Roy et al, 2015; Erkel, 2009).

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