Abstract

The hypothesis that residual cyanide present in cassava flour influences the growth of mycotoxigenic Aspergillus was established. Therefore, cyanide concentrations were measured in three types of flour: mixed, dry and watery (n=30), as the basis for the establishment of doses to be used. Fungi sowing were performed in solid Saboraud medium with a central well (500 μL), in which 5, 10, 15 and 20 mg CN kg-1 concentrations were applied, and control group was established using distilled water (0 mg CN kg-1). Plates were incubated at 25°C for 24, 48 and 120 h and evaluated on direct observation of fungal growth. Results showed that fungi grown unevenly according to the applied concentration. The initial development at all concentrations, in both genera, was by the edge of plates, at a slower rate in larger doses. After 120 h only the 20 mg kg-1 concentration did not grow near the center circle. Thus, results show that cyanide slows fungi growth of mycotoxigenic Aspergillus flavus assessed, with dose-dependent effect. It is important to apply the good practices in cassava flour production in order to control cyanide levels, as well as the reduction of toxigenic fungi, to promote the food safety. Key words: Mycotoxin, linamarin, Manihot, Aspergillus.

Highlights

  • Cassava (Manihot esculenta Crantz) is part of the food base and cuisine of Latin America and Africa

  • The hypothesis that residual cyanide present in cassava flour influences the growth of mycotoxigenic Aspergillus was established

  • Results show that cyanide slows fungi growth of mycotoxigenic Aspergillus flavus assessed, with dosedependent effect

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Summary

Introduction

Cassava (Manihot esculenta Crantz) is part of the food base and cuisine of Latin America and Africa. The flour is of great prominence due to the heterogeneity of manufacturing processes, resulting in typical products of each region. In the Amazon region (northern Brazil), the main type of flour used is the watery. This type of flour is produced through an empirical process of roots fermentation, followed by pressing, grating and roasting (Cereda and Vilpoux, 2010; Chisté and Cohen, 2011). This product is available heterogeneously in the market due to a large physicochemical and microbiological variation

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